Human protective response induced by meningococcus B vaccine is mediated by the synergy of multiple bactericidal epitopes

Scientific Reports
M GiulianiVega Masignani

Abstract

4CMenB is the first broad coverage vaccine for the prevention of invasive meningococcal disease caused by serogroup B strains. To gain a comprehensive picture of the antibody response induced upon 4CMenB vaccination and to obtain relevant translational information directly from human studies, we have isolated a panel of human monoclonal antibodies from adult vaccinees. Based on the Ig-gene sequence of the variable region, 37 antigen-specific monoclonal antibodies were identified and produced as recombinant Fab fragments, and a subset also produced as full length recombinant IgG1 and functionally characterized. We found that the monoclonal antibodies were cross-reactive against different antigen variants and recognized multiple epitopes on each of the antigens. Interestingly, synergy between antibodies targeting different epitopes enhanced the potency of the bactericidal response. This work represents the first extensive characterization of monoclonal antibodies generated in humans upon 4CMenB immunization and contributes to further unraveling the immunological and functional properties of the vaccine antigens. Moreover, understanding the mechanistic nature of protection induced by vaccination paves the way to more rational vacc...Continue Reading

References

Jun 5, 2002·The Journal of Experimental Medicine·Maurizio ComanducciMarirosa Mora
Mar 19, 2003·The Journal of Experimental Medicine·Vega MasignaniMariagrazia Pizza
Mar 25, 2004·Infection and Immunity·Leah D FletcherGary W Zlotnick
Jun 24, 2004·Infection and Immunity·Maurizio ComanducciMariagrazia Pizza
Dec 13, 2005·Analytical Biochemistry·Robert KarlssonDavid G Myszka
Jul 3, 2007·Lancet·David S StephensPetter Brandtzaeg
Feb 27, 2008·Journal of Leukocyte Biology·Susanna FranzosoEmanuele Papini
Dec 24, 2008·The Journal of Biological Chemistry·Alessandro MascioniDésirée H H Tsao
Feb 7, 2009·The Journal of Biological Chemistry·Francesca CantiniRino Rappuoli
Apr 24, 2009·Vaccine·Seppo MeriHanna Jarva
Oct 20, 2009·Biochimica Et Biophysica Acta·Alessandro MascioniDésirée H H Tsao
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Davide SerrutoBeatrice Aricò
Jun 29, 2010·Molecular Immunology·Viviana P FerreiraClaudio Cortés
Nov 19, 2010·Bioanalysis·Johanna R MoraVimal Vimal Patel
May 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Laura CendronGiuseppe Zanotti
Feb 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Enrico MalitoVega Masignani
Dec 29, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Agnese FaleriVega Masignani
May 9, 2014·Clinical and Vaccine Immunology : CVI·Stefania BambiniKeith A Jolley
Nov 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Enrico MalitoMatthew James Bottomley
Feb 19, 2016·Proceedings of the National Academy of Sciences of the United States of America·Massimiliano BiaginiVega Masignani
Sep 9, 2016·BMJ : British Medical Journal·Jacqui Wise

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Citations

Nov 28, 2018·Nature Reviews. Immunology·Maria GeorgievaMarc Lipsitch
Nov 21, 2018·Expert Review of Vaccines·Rino RappuoliKumaran Vadivelu
Aug 24, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Federica BianchiRoberta Cozzi
Oct 1, 2019·Current Opinion in Infectious Diseases·Robert C Read
Jul 30, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eriberto Noel NataliMaria Scarselli
Apr 17, 2020·FEBS Letters·Silvia PrincipatoRino Rappuoli
Aug 28, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shamez N LadhaniMary E Ramsay
Jan 20, 2021·NPJ Vaccines·Alexander KlimkaMartin Krönke
Mar 9, 2021·Journal of the American Society for Mass Spectrometry·Laura R GrauslundKasper D Rand
Jul 15, 2021·Computational and Structural Biotechnology Journal·Sonia BudroniOretta Finco
Jul 22, 2021·Expert Review of Vaccines·Jamie FindlowPaul Balmer
Oct 31, 2021·NPJ Vaccines·Yara Ruiz GarcíaRafik Bekkat-Berkani

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Datasets Mentioned

BETA
GSE98883

Methods Mentioned

BETA
nuclear magnetic resonance
flow cytometry
Protein chip
chip
Surface Plasmon Resonance
Assay
Protein
protein array
PCR
protein assay

Software Mentioned

R
PowerScanner
ImaGene
PepScan
GyroLab
Biacore T200 Evaluation

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