Human pulmonary endothelial cells in culture. Activities of cells from arteries and cells from veins

The Journal of Clinical Investigation
A R Johnson

Abstract

Endothelial cells were cultured from various different human vessels, including aortas, pulmonary, ovarian, and umbilical arteries, and pulmonary, ovarian, and umbilical veins. The cultured cells were identified as endothelial cells by the presence of Factor VIII antigen and antiotensin I converting enzyme (kininase II). They retained these markers for at least five passages in culture, and some cells had them for seven passages or more. Endothelial cells from the various vessels were compared with respect to their ability to metabolize angiotensins I and II and bradykinin. Cells from arteries had three to five times the angiotensin I converting enzyme activity as cells from veins. The activity of angiotensinase A (aspartyl aminopeptidase) had a similar distribution, and cells from arteries were consistently more active than cells from veins. Cultures of endothelial cells from pulmonary and umbilical vessels formed prostacyclin in response to mechanical stimulation. Media from cell monolayers that were subjected to a change of medium and gentle agitation inhibited aggregation of human platelets. This inhibitory activity was generated within 2-5 min, and it was not formed by cells that were treated with indomethacin or tranylcyp...Continue Reading

References

Mar 12, 1976·Science·P R CaldwellR L Soffer
Jun 29, 1978·Biochemical and Biophysical Research Communications·L W HayesL L Slakey
May 1, 1979·The Journal of Clinical Investigation·S A StalcupR B Mellins
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F M BooyseE J Bowie
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B B WekslerE A Jaffe
Nov 1, 1978·The Journal of Clinical Investigation·B B WekslerE A Jaffe
Sep 1, 1978·Proceedings of the Society for Experimental Biology and Medicine·P J LeuenbergerG M Turino
Sep 9, 1977·Biochemical and Biophysical Research Communications·N L BaenzigerP W Majerus
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D GospodarowiczC Birdwell
Jan 1, 1975·Journal of Ultrastructure Research·C C HaudenschildJ Folkman
Jun 1, 1975·Circulation Research·D SheproF A Belamarich
Nov 1, 1973·The Journal of Clinical Investigation·L W HoyerJ R Hoyer
Oct 1, 1969·The American Journal of Cardiology·P BironP David
Sep 1, 1968·Life Sciences·P Biron, C G Huggins
Mar 1, 1974·The Journal of Cell Biology·M A GimbroneJ Folkman
Feb 1, 1969·British Journal of Pharmacology·J R Vane
Nov 1, 1973·The Journal of Clinical Investigation·E A JaffeR L Nachman
Aug 3, 1973·Science·L J LewisG L Fry

Citations

Mar 1, 1990·Journal of Cellular Physiology·Y SaegusaD E Cavender
Feb 1, 1990·Cancer Metastasis Reviews·P N Belloni, R J Tressler
Feb 1, 1992·Diabetologia·M Z Gilcrease, R L Hoover
Sep 1, 1985·Basic Research in Cardiology·K Schrör
Jan 1, 1987·Annals of Biomedical Engineering·C N Gillis
Apr 1, 1984·In Vitro·O TokunagaT Nakashima
Sep 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R Weinstein, K Wenc
Jun 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M P Carson, C C Haudenschild
May 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K J EtchbergerM B Herring
Feb 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D B Stolz, B S Jacobson
Nov 1, 1993·In Vitro Cellular & Developmental Biology. Animal·P W Hewett, J C Murray
Jan 1, 1986·Lung·S P Hanley
Jan 1, 1981·Lung·J A Pang, D M Geddes
Oct 1, 1982·In Vitro·M K GlassbergB R Zetter
Jan 24, 1992·Biochimica Et Biophysica Acta·G E Revtyak, W B Campbell
Oct 10, 1989·European Journal of Pharmacology·O F Cole, G P Lewis
May 1, 1987·Journal of Steroid Biochemistry·L MilewichA R Johnson
Nov 1, 1985·Prostaglandins, Leukotrienes, and Medicine·S P Hanley, J Bevan
Aug 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·O ChappeyJ L Wautier
Mar 1, 1997·The Annals of Thoracic Surgery·G PompilioA Sala
Feb 25, 2000·Thrombosis Research·M Spadafora-FerreiraJ Kalil
Nov 8, 2011·Chemical Research in Toxicology·Tessa J Barrett, Clare L Hawkins
Oct 28, 1982·The New England Journal of Medicine·A C CarvalhoW M Zapol
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R AuerbachS L Watt
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R L HooverR A Lewis
Nov 1, 1981·The Journal of Cell Biology·T MaciagR Weinstein
Apr 1, 1986·Annals of Surgery·V J HendersonD C Miller
May 1, 1983·British Journal of Haematology·C WillemsJ A van Mourik
Sep 1, 1981·Clinical and Experimental Pharmacology & Physiology·F A Mendelsohn, C Kachel
Apr 1, 1987·Pediatrics·A M Mauer
Mar 31, 2012·American Journal of Physiology. Renal Physiology·Juan Carlos Q VelezMichael G Janech
Mar 20, 2012·American Journal of Respiratory Cell and Molecular Biology·Suzy A A ComhairSerpil C Erzurum
Jan 1, 1981·The Journal of Clinical Investigation·K KumamotoE G Erdös
Apr 18, 2009·Anesthesia and Analgesia·Kenichi A TanakaJerrold H Levy
Jan 25, 2008·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Yuichiro HataHiroyuki Ishikawa
Nov 4, 2004·Biochemical and Biophysical Research Communications·Yoshimi NakagawaHitoshi Miyazaki
Jan 1, 1997·Endothelium : Journal of Endothelial Cell Research·B BaudinL Drouet
Apr 3, 2014·IEEE Transactions on Bio-medical Engineering·Elizabeth A CalleLaura E Niklason
Jan 1, 1983·Thrombosis Research·J M HarlanL J Weaver
Jul 1, 1988·Thrombosis Research·J G GarciaA R Johnson
Jun 3, 2014·Head & Face Medicine·Tobias AnnussekKai Wermker
Oct 16, 2002·Biochimica Et Biophysica Acta·S MillerG J Beckett
Jun 22, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·T Bachetti, L Morbidelli
Jul 1, 1983·Microvascular Research·J D Gitlin, P A D'Amore
Jul 16, 1985·Biochemical and Biophysical Research Communications·Y InadaY Saito
Mar 31, 1981·Biochemical and Biophysical Research Communications·W SiessF Russo-Marie
Mar 1, 1984·Experimental Cell Research·P T PiccianoD L Kreutzer
Nov 21, 2012·Journal of Tissue Engineering and Regenerative Medicine·I L A GeenenM J Post
May 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J I FannD C Miller
May 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J A BerlinerA M Fogelman
Mar 1, 1983·Stroke; a Journal of Cerebral Circulation·S Moncada
Nov 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·H SageP Bornstein
Nov 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·V W van HinsberghM Scheffer
Sep 1, 1982·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T L KaduceR S Bar
May 1, 1982·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S Moncada
Jan 1, 1982·Annals of the New York Academy of Sciences·L Reid, B Meyrick
Jan 1, 1982·Annals of the New York Academy of Sciences·A F Junod
Jan 1, 1982·Annals of the New York Academy of Sciences·E A Jaffe
Sep 1, 1981·Journal of Cellular Physiology·P J Del Vecchio, J R Smith
Apr 1, 1987·Journal of Cellular Physiology·J P NoveralE M Levine
Sep 1, 1996·In Vitro Cellular & Developmental Biology. Animal·P W Hewett, J C Murray
Jan 1, 1991·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·Y SasaguriM Morimatsu
May 9, 2014·Journal of the American Society of Nephrology : JASN·Raymond VanholderGriet Glorieux
Aug 23, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dmitry KondrikovYunchao Su
Apr 11, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ian B CoplandMartin Post
Sep 11, 2019·International Journal of Molecular Sciences·Anne Krüger-GengeFriedrich Jung
Jul 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R G ShawB Combes
Jan 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·J A WhitneyP W Shaul
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·A Heifetz, A R Johnson
Mar 1, 2003·American Journal of Physiology. Heart and Circulatory Physiology·M Ursula NormanRebecca A Lew
Mar 3, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Willeke de HaanAernout Luttun
Aug 1, 1988·The American Journal of Physiology·G E RevtyakW B Campbell
Jan 1, 1988·The American Journal of Physiology·G E RevtyakW B Campbell

Related Concepts

Antigens
Ascending Aorta Structure
Arteries
Endothelium
Factor VIII Procoagulant Activity
Lung
Ovary
Platelet Aggregation
Prostaglandins
Flolan

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.