Human pulp-derived cells immortalized with Simian Virus 40 T-antigen

European Journal of Oral Sciences
K GallerGottfried Schmalz

Abstract

Primary cells in culture have a limited capacity to divide and soon reach a non-proliferative state. This cellular senescence limits the investigation of cells derived from human pulp concerning cellular pathways, gene regulation, mechanisms of dentin formation, or responses to material exposure. To overcome this problem, primary human pulp-derived cells were established and transfected with a plasmid containing coding sequences of Simian Virus 40 (SV40) large T-antigen. This resulted in the establishment of several cell clones showing an extension of life span. Expression of T-antigen transcripts and protein was verified by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Primary human pulp cells were cultured until senescence (i.e. up to passage 7) and transfected cells could be cultured to passage 18 after transfection, when a cellular crisis with massive cell death occurred. One clone escaped from crisis and has been maintained in culture for 55 wk. Experiments were performed to characterize transfected cells in comparison to primary cells. Cell morphology and proliferation were analyzed, and expression of cell-specific gene transcripts and proteins (including collagen types I and III, alka...Continue Reading

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Citations

Jan 19, 2011·Clinical Oral Investigations·Stephanie KrifkaHelmut Schweikl
Feb 1, 2012·Clinical Oral Investigations·Hayriye Esra ÜlkerGottfried Schmalz
Feb 18, 2011·Protein Expression and Purification·Hisashi SuguroKazuo Komiyama
Nov 17, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Carlos Henrique Ribeiro CamargoHelmut Schweikl
Feb 21, 2009·International Endodontic Journal·S E A CamargoG Schmalz
May 16, 2008·International Endodontic Journal·H SuguroK Komiyama
Mar 14, 2007·Archives of Oral Biology·Masae KitagawaTakashi Takata
Jun 1, 2015·Dental Materials : Official Publication of the Academy of Dental Materials·Kirsten L Van LanduytHelmut Schweikl
Jun 9, 2009·Biochemical and Biophysical Research Communications·Li-An WuShuo Chen
Jul 28, 2007·Dental Materials : Official Publication of the Academy of Dental Materials·M DemirciH Schweikl
Jan 26, 2010·Biomaterials·Will W MinuthAnne Glashauser
Feb 18, 2017·International Endodontic Journal·M WidbillerK M Galler
Jun 17, 2011·Advances in Dental Research·G SchmalzH Schweikl
Dec 8, 2010·Journal of Dental Research·K M GallerG Schmalz
Feb 28, 2015·The Journal of Advanced Prosthodontics·Meral Arslan MalkoçSema Sezgin Hakki
Feb 6, 2019·International Journal of Molecular Sciences·Takeo KarakidaYasuo Yamakoshi
Jun 15, 2013·Macromolecular Bioscience·Obi EgbuniweLucy Di Silvio
May 26, 2017·Tissue Engineering. Part a·Kerstin M GallerGottfried Schmalz
Apr 11, 2018·PloS One·Fernando Freitas de LimaEliana Janet Sanjinez-Argandoña
Aug 22, 2018·International Journal of Molecular Sciences·Shunjiro YamakawaNoriyasu Hosoya

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