PMID: 8614565Nov 17, 1995Paper

Human ramified microglial cells produce nitric oxide upon Escherichia coli lipopolysaccharide and tumor necrosis factor alpha stimulation

Neuroscience Letters
M ColasantiG M Lauro

Abstract

This study shows that human ramified microglial cells derived from fetal brain primary cultures, are able to produce nitric oxide (NO). In fact, stimulation with Escherichia coli lipopolysaccharide (LPS) (1 microgram ml-1) or tumor necrosis factor alpha (TNF alpha) (500 U ml-1) enhances nitrite release in cell supernatants, as determined by the Griess reaction. A synergistic effect is achieved following treatment with LPS plus TNF alpha, this effect being inhibited by pretreating cells with NOS inhibitor N omega-nitro-L-arginine methyl ester (L-NAME). Using reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blot analysis, we also found that LPS/TNF alpha produce an increase of inducible NO synthase (iNOS) mRNA expression.

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Citations

Jun 18, 2002·Journal of Neurochemistry·Benoît ChénaisJean-Claude Drapier
Oct 29, 1998·Mediators of Inflammation·V A VincentA M Van Dam
Oct 6, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R PaulH W Pfister
Apr 1, 2014·Molecular Medicine Reports·Da-Wei LiGuang-Ren Li
Jul 14, 2006·Journal of Neurochemistry·Fabio BiancoClaudia Verderio
Jan 7, 2014·Trends in Neurosciences·Amy M Smith, Mike Dragunow
Jan 5, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R GregersenB Finsen
Jan 19, 2006·Journal of Child Neurology·Rebecca D Folkerth
Sep 29, 2006·Glia·Katrin Färber, Helmut Kettenmann
Jun 11, 2005·Journal of Neuroscience Research·Carolyn JackJack P Antel
Oct 4, 2005·Brain Pathology·R L HaynesD R Folkerth
Nov 10, 1995·The Journal of Biological Chemistry·M ColasantiH Suzuki
Oct 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mei-Jen WangWu-Fu Chen
Dec 31, 2004·Journal of Neuropathology and Experimental Neurology·Eleanor S RobertsHoward S Fox
Feb 16, 1999·Brain Pathology·K J SmithP A Felts
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nazila Janabi
Oct 20, 1998·Journal of Neuroscience Research·S ElkabesI B Black
Nov 22, 1997·Brain Research·J L GoodwinE Uemura
Jan 1, 1996·Advances in Neuroimmunology·J Zielasek, H P Hartung
Jun 17, 2006·Biochimica Et Biophysica Acta·Jana MehlhaseTilman Grune

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