Human RASSF7 regulates the microtubule cytoskeleton and is required for spindle formation, Aurora B activation and chromosomal congression during mitosis.

The Biochemical Journal
Asha RecinoAndrew D Chalmers

Abstract

RASSF7, a member of the N-terminal Ras association domain family, has increased expression in various cancers and, on the basis of our previous work in Xenopus embryos, may be a regulator of mitosis. In the present study, we address, for the first time, the role of human RASSF7 in mitosis. We demonstrate that RASSF7 is expressed in a broad range of different cell types and that this expression could be enhanced following exposure to hypoxia. Knocking down RASSF7 in human cell lines inhibited cell growth and induced defects in mitosis, including aberrant spindle formation and a failure in chromosomal congression. In order to understand the molecular basis of the defects in more detail, we analysed the activity of mitotic signalling proteins and found that activation of Aurora B did not occur in cells in which RASSF7 was knocked down. We also show that endogenous RASSF7 protein localizes to the centrosome and demonstrate using microtubule-regrowth assays that RASSF7 is an important regulator of microtubule dynamics. On the basis of these observations, we propose that, owing to its key role in regulating the microtubule cytoskeleton, RASSF7 is required for mitosis in human cells.

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Citations

Aug 21, 2013·Advances in Biological Regulation·Jia Jia Chan, Matilda Katan
Feb 12, 2014·Journal of Cancer Research and Therapeutics·Xi LiShijie Xin
Mar 13, 2014·FEBS Letters·Natalia VolodkoShairaz Baksh
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Mar 15, 2012·The Prostate·Temuujin DansranjavinUndraga Schagdarsurengin
Aug 25, 2018·The Journal of Biological Chemistry·Anbarasu KumaraswamySundarasamy Mahalingam
Oct 27, 2018·Journal of Cellular Physiology·Zhiyun HeZhongtao Zhang
Jul 28, 2017·Journal of the American Society of Nephrology : JASN·Jesus H DominguezK J Kelly
Feb 28, 2014·Oncology Reports·Zhenhua LiQiang Sun
Dec 1, 2010·Epigenetics : Official Journal of the DNA Methylation Society·Nicholas Underhill-DayFarida Latif
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Naheed Arfin Borah, Mamatha M Reddy
Aug 4, 2021·Cell Death Discovery·Jun YuanJiahai Shi

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