Abstract
We previously observed that human, recombinant interleukin-2 in a pharmacologic dose (200 u/ml) induced histamine release from monocyte-depleted peripheral blood mononuclear cells in vitro. Therefore, we studied the role of various pharmacologic doses of rIL-2 on in vitro histamine release. Peripheral blood mononuclear cells (5 x 10(6) cells/ml), which also contain basophils, from 13 patients scheduled for elective colorectal cancer surgery and 10 age and sex matched healthy volunteers were stimulated with rIL-2 in concentrations of 0, 50, 100, 200, 450, 900, 1,800 and 3,600 u/ml, respectively, for 1, 24 and 48 hours under standard conditions. Histamine was analysed in supernatants using the glass fiber method. Simultaneously, total cell-bound histamine was analysed in lysate from 5 x 10(6) mononuclear cells from all patients and volunteers, thus allowing determination of percent histamine release. Supernatant histamine concentration from unstimulated cells was 17.2 +/- 1.5 ng/ml in patients compared to 7.9 +/- 1.0 ng/ml in volunteers (#p < 0.05) after 1 hour stimulation, and no further increase was observed after 24 and 48 hours, respectively. Histamine concentration increased significantly in the supernatant from cells stimul...Continue Reading
References
Jan 1, 1992·International Archives of Allergy and Immunology·M ShibataD Morton
Aug 1, 1991·Annals of Surgery·R WelbournH B Hechtman
Nov 15, 1991·Cancer·S FujitaV J Ferrans
Jun 1, 1991·The Journal of Surgical Research·M J EdwardsF N Miller
Apr 5, 1990·The New England Journal of Medicine·M S KlempnerM B Atkins
Nov 1, 1990·The Journal of Allergy and Clinical Immunology·M AnderssonU Pipkorn
Feb 1, 1987·Allergy·Y MoritaT Miyamoto
Feb 29, 1988·Biochemical and Biophysical Research Communications·R J WhiteheadD E Woolley
Apr 9, 1987·The New England Journal of Medicine·W H WestR K Oldham
Apr 1, 1984·Agents and Actions·P Stahl SkovB Weeke
Jul 1, 1982·The Journal of Investigative Dermatology·S H PincusW R Schooley
Jan 1, 1993·Cancer Biotherapy·P A PalmerR Oskam
Nov 1, 1994·Gut·W J AdamsD L Morris
Aug 1, 1994·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J C YangH I Kim
Jan 1, 1993·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A Lindemann, R Mertelsmann
Mar 23, 1994·JAMA : the Journal of the American Medical Association·S A RosenbergD E White
Mar 1, 1994·The Journal of Allergy and Clinical Immunology·W D DachmanB B Hoffman
Aug 1, 1993·Gut·S A WatsonJ D Hardcastle
Sep 1, 1993·Immunopharmacology·B Delaney, N E Kaminski
Jan 28, 1993·The New England Journal of Medicine·S J Galli
Feb 1, 1993·The British Journal of Surgery·S D HeysO Eremin
Apr 1, 1993·Cancer·R O DillmanR Birch