Human recombinant pro-dipeptidyl peptidase I (cathepsin C) can be activated by cathepsins L and S but not by autocatalytic processing

Biochemistry
S W DahlB Turk

Abstract

Human dipeptidyl peptidase I was expressed in the insect cell/baculovirus system and purified in its active (rhDPPI) and precursor (pro-rhDPPI) forms. RhDPPI was very similar to the purified enzyme (hDPPI) with respect to glycosylation, enzymatic processing, oligomeric structure, CD spectra, and catalytic activity. The precursor, which was a dimer, could be activated approximately 2000-fold with papain. Cathepsin L efficiently activated pro-rhDPPI in vitro at pH 4.5 (k(app) approximately 2 x 10(3) min(-)(1) M(-)(1)), and two cleavage pathways were characterized. The initial cleavage was within the pro region between the residual pro part and the activation peptide. Subsequently, the activation peptide was cleaved from the catalytic region, and the latter was cleaved into the heavy and light chains. Alternatively, the pro region was first separated from the catalytic region. Cathepsin S was a less efficient activating enzyme. Cathepsin B and rhDPPI did not activate pro-rhDPPI, and the proenzyme was incapable of autoactivation. Incubation of both pro-rhDPPI and rhDPPI with cathepsin D resulted in degradation. Cystatin C and stefins A and B inhibited rhDPPI with K(i) values in the nanomolar range (K(i) = 0.5-1.1 nM). The results s...Continue Reading

References

Feb 15, 1992·European Journal of Biochemistry·T NikawaN Katunuma
Jul 1, 1970·European Journal of Biochemistry·S BlumbergA Berger
Sep 15, 1995·The Journal of Biological Chemistry·I DolencV Turk
Oct 3, 1994·FEBS Letters·K Ishidoh, E Kominami
Dec 1, 1994·American Journal of Respiratory and Critical Care Medicine·G H Caughey
Sep 2, 1996·FEBS Letters·I DolencV Turk
May 23, 1997·The Journal of Biological Chemistry·M S McQueneyC S Jones
Feb 1, 1996·Protein Expression and Purification·K SakaiL B Schwartz
Mar 21, 1998·Biochimica Et Biophysica Acta·B CigićR H Pain
Jun 23, 1998·The Journal of Biological Chemistry·P J WoltersG H Caughey
Sep 17, 1998·European Journal of Biochemistry·L Hola-JamriskaP J Brindley
Feb 5, 2000·American Journal of Respiratory Cell and Molecular Biology·P J WoltersG H Caughey

❮ Previous
Next ❯

Citations

Dec 31, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ke-Jin Hu, Pak-Chow Leung
Jul 19, 2002·Advances in Enzyme Regulation·Vito TurkJanko Kos
Dec 31, 2003·Methods : a Companion to Methods in Enzymology·Dieter BrömmeBoris Turk
Sep 5, 2001·The EMBO Journal·V TurkD Turk
Mar 23, 2002·Protein Science : a Publication of the Protein Society·Martin HornMichael Mares
Jan 15, 2013·BMC Medical Genetics·José G Romero-QuintanaRosalío Ramos-Payán
Nov 20, 2002·Biological Chemistry·Boris TurkVito Turk
Jul 31, 2003·Biological Chemistry·Boris Turk, Hans Fritz
Aug 10, 2006·Biological Chemistry·Jon Mallen-St ClairPaul J Wolters
Feb 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei WangJianghui Xie
Jul 10, 2014·Acta Crystallographica. Section D, Biological Crystallography·William J BradshawK Ravi Acharya
Sep 15, 2011·Biochimica Et Biophysica Acta·Urška RepnikBoris Turk
Oct 26, 2011·Biochimica Et Biophysica Acta·Vito TurkDušan Turk
Sep 26, 2013·Genes & Development·Brian RuffellLisa M Coussens
Jan 15, 2011·Trends in Cell Biology·Steven D Mason, Johanna A Joyce
Sep 14, 2010·Molecular and Biochemical Parasitology·Flora WangMichael Klemba
Sep 27, 2012·Biochemistry·Maria M ReifChris Oostenbrink
May 13, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Lihua QiuKeng Yang
Sep 27, 2005·Immunological Reviews·Lianne C Hsing, Alexander Y Rudensky
Nov 17, 2009·Immunological Reviews·Stephan HailfingerMargot Thome
Apr 24, 2004·Trends in Parasitology·Conor R CaffreyMohammed Sajid
Apr 1, 2014·Biochimica Et Biophysica Acta·Marko Fonović, Boris Turk
May 2, 2006·Bioorganic & Medicinal Chemistry Letters·Jon BondebjergLars Naerum
May 17, 2005·Bioorganic & Medicinal Chemistry·Jon BondebjergLars Naerum
Mar 21, 2015·World Journal of Microbiology & Biotechnology·Chunfang LingAilin Tao
Aug 26, 2014·Analytical Biochemistry·Monika LęgowskaAdam Lesner
May 21, 2016·Frontiers in Pharmacology·Sonia VermaKailash C Pandey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.