Human resident gut microbe Bacteroides thetaiotaomicron regulates colonic neuronal innervation and neurogenic function.

Gut Microbes
Rubina AktarMadusha Peiris

Abstract

As the importance of gut-brain interactions increases, understanding how specific gut microbes interact with the enteric nervous system (ENS), which is the first point of neuronal exposure becomes critical. Our aim was to understand how the dominant human gut bacterium Bacteroides thetaiotaomicron (Bt) regulates anatomical and functional characteristics of the ENS. Neuronal cell populations, as well as enteroendocrine cells, were assessed in proximal colonic sections using fluorescent immunohistochemistry in specific pathogen-free (SPF), germ-free (GF) and Bt conventionalized-germ-free mice (Bt-CONV). RNA expression of tight junction proteins and toll-like receptors (TLR) were measured using qPCR. Colonic motility was analyzed using in vitro colonic manometry. Decreased neuronal and vagal afferent innervation observed in GF mice was normalized by Bt-CONV with increased neuronal staining in mucosa and myenteric plexus. Bt-CONV also restored expression of nitric oxide synthase expressing inhibitory neurons and of choline acetyltransferase and substance P expressing excitatory motor neurons comparable to those of SPF mice. Neurite outgrowth and glial cells were upregulated by Bt-CONV. RNA expression of tight junction protein claud...Continue Reading

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Citations

Nov 27, 2020·Frontiers in Microbiology·Amisha ModasiaSimon R Carding
Jan 27, 2021·Journal of Experimental & Clinical Cancer Research : CR·Yanshan GeMinghua Wu
Oct 24, 2020·Nature Reviews. Microbiology·Livia H MoraisSarkis K Mazmanian
Feb 27, 2021·The International Journal of Biochemistry & Cell Biology·Julie E DalzielWayne Young
Dec 15, 2020·Trends in Microbiology·Amélie JolyFilipe De Vadder
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hyemee KimSusanne U Mertens-Talcott
Jun 15, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Candice FungPieter Vanden Berghe
Jul 13, 2021·Frontiers in Behavioral Neuroscience·Alina WilkowskaWiesław Jerzy Cubała

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Methods Mentioned

BETA
PCR
reverse transcription PCR

Software Mentioned

Medical Measurement System ( MMS )
Image J
GraphPad
GraphPad Prism
JACoP
Metamorph

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