Human retinal pigment epithelium proteome changes in early diabetes.

Diabetologia
A DecaniniDeborah A Ferrington

Abstract

Diabetic retinopathy is the most common complication of diabetes and a leading cause of blindness among working-age adults. Anatomical and functional changes occur in the retina and retinal pigment epithelium (RPE) prior to clinical symptoms of the disease. However, the molecular mechanisms responsible for these early changes, particularly in the RPE, remain unclear. To begin defining the molecular changes associated with pre-retinopathic diabetes, we conducted a comparative proteomics study of human donor RPE. The RPE was dissected from diabetic human donor eyes with no clinically apparent diabetic retinopathy (n=6) and from eyes of age-matched control donors (n=17). Soluble proteins were separated based upon their mass and charge using two-dimensional (2-D) gel electrophoresis. Protein spots were visualised with a fluorescent dye and spot densities were compared between diabetic and control gels. Proteins from spots with significant disease-related changes in density were identified using mass spectrometry. Analysis of 325 spots on 2-D gels identified 31 spots that were either up- or downregulated relative to those from age-matched control donors. The protein identity of 18 spots was determined by mass spectrometry. A majorit...Continue Reading

References

Oct 15, 1985·Documenta Ophthalmologica. Advances in Ophthalmology·R H Steinberg
Apr 1, 1997·Cardiovascular Research·W C StanleyJ G McCormack
Aug 1, 1997·Neurochemical Research·W G McLean
Jan 20, 1999·Cardiovascular Research·N S DhallaN Takeda
Nov 16, 2001·American Journal of Physiology. Heart and Circulatory Physiology·I V TurkoF Murad
Jan 5, 2002·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·T Bensaoula, A Ottlecz
May 29, 2002·Diabetes·Raghavakaimal SreekumarK Sreekumaran Nair
Jul 20, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Toyofumi NakanishiAkira Shimizu
Nov 6, 2002·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Yoshinori AizuHachiro Nakagawa
Feb 25, 2003·Progress in Retinal and Eye Research·A N WitmerR O Schlingemann
Feb 26, 2003·Molecular & Cellular Proteomics : MCP·Karen A WestJohn W Crabb
Mar 11, 2003·American Journal of Physiology. Endocrinology and Metabolism·Gregory J CrowtherKevin E Conley
Sep 17, 2003·Molecular & Cellular Proteomics : MCP·Ken YamaneHiromu K Mishima
Nov 6, 2003·Redox Report : Communications in Free Radical Research·David M van ReykMichael J Davies
Dec 24, 2003·Biochemistry·Rebecca J KapphahnDeborah A Ferrington
Sep 29, 2004·Investigative Ophthalmology & Visual Science·Kristian KlempHenrik Lund-Andersen
Nov 24, 2004·Investigative Ophthalmology & Visual Science·Timothy W Olsen, Xiao Feng
Nov 24, 2004·Investigative Ophthalmology & Visual Science·Joanna A PhippsAlgis J Vingrys
Mar 22, 2005·Protein and Peptide Letters·Júlio C Borges, Carlos H I Ramos
Aug 6, 2005·Experimental Eye Research·Masayuki OuchiMotohiro Kamei
Dec 29, 2005·American Journal of Physiology. Endocrinology and Metabolism·M Shamsul OlaKathryn F LaNoue
Mar 1, 2006·Investigative Ophthalmology & Visual Science·Curtis L NordgaardDeborah A Ferrington
May 26, 2006·Investigative Ophthalmology & Visual Science·Cheryl M EthenDeborah A Ferrington
Jul 11, 2006·The Journal of Clinical Endocrinology and Metabolism·Lucia PuricelliPaolo Tessari
Aug 29, 2006·Diabetes·David A AntonettiUNKNOWN JDRF Diabetic Retinopathy Center Group
Oct 27, 2006·Investigative Ophthalmology & Visual Science·David J RamseyHaohua Qian
Feb 10, 2007·The FEBS Journal·Hiderou Yoshida
Jun 16, 2007·Pharmacology & Therapeutics·Elizabeth M Ellis

❮ Previous
Next ❯

Citations

Oct 1, 2013·Diabetologia·Alice LyMarius Ueffing
Jun 1, 2011·Journal of Ocular Biology, Diseases, and Informatics·Hui-Zhuo XuYun-Zheng Le
Nov 22, 2011·Journal of Proteome Research·Alice C L LenMark C Gillies
Apr 30, 2010·PloS One·Lan WangChristine A Curcio
Dec 17, 2014·Bioanalysis·Lasse Jørgensen CehofskiHenrik Vorum
Sep 13, 2012·Current Eye Research·Karunakaran CoralNarayanasamy Angayarkanni
Dec 15, 2015·The Protein Journal·Srinivas R SripathiWan Jin Jahng
Apr 25, 2009·Clinics in Laboratory Medicine·Michael L Merchant, Jon B Klein
Mar 8, 2012·Clinical & Experimental Ophthalmology·Narelle L Jay, Mark Gillies
Jun 12, 2013·Proteomics·Richard D SembaJennifer E Van Eyk
Apr 8, 2016·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Eui Chun KangSuk Ho Byeon
Dec 4, 2014·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Heba Al-Hussaini, Narayana Kilarkaje
Jun 13, 2015·Journal of Ophthalmology·Lasse Jørgensen CehofskiHenrik Vorum
Dec 3, 2014·Diabetes & Metabolic Syndrome·Haseeb Ahsan
Apr 30, 2017·International Journal of Molecular Sciences·Lasse Jørgensen CehofskiHenrik Vorum
Jan 23, 2018·Proteomics·Meleha T AhmadRichard D Semba
Jul 22, 2016·American Journal of Physiology. Cell Physiology·Mitra FarnoodianNader Sheibani
May 29, 2020·Indian Journal of Ophthalmology·K DivyaD Sundar
Sep 19, 2019·Investigative Ophthalmology & Visual Science·Jacqueline M Lopes de FariaJosé B Lopes de Faria
May 31, 2018·Investigative Ophthalmology & Visual Science·Jeffrey M SundstromRafael Simó
Oct 29, 2020·Journal of Diabetes Research·Ioana Damian, Simona Delia Nicoara
Feb 2, 2021·Journal of Diabetes and Metabolic Disorders·Ambrose Teru PatrickWan Jin Jahng
Mar 23, 2021·Investigative Ophthalmology & Visual Science·Shuxin FanXialin Liu
Apr 24, 2021·World Journal of Diabetes·Ştefan Ţălu, Simona Delia Nicoara
Nov 16, 2010·Journal of Proteomics·Hilal ArnoukWan Jin Jahng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.