Human Rev1 polymerase disrupts G-quadruplex DNA

Nucleic Acids Research
Sarah EddyRobert L Eoff

Abstract

The Y-family DNA polymerase Rev1 is required for successful replication of G-quadruplex DNA (G4 DNA) in higher eukaryotes. Here we show that human Rev1 (hRev1) disrupts G4 DNA structures and prevents refolding in vitro. Nucleotidyl transfer by hRev1 is not necessary for mechanical unfolding to occur. hRev1 binds G4 DNA substrates with Kd,DNA values that are 4-15-fold lower than those of non-G4 DNA substrates. The pre-steady-state rate constant of deoxycytidine monophosphate (dCMP) insertion opposite the first tetrad-guanine by hRev1 is ∼56% as fast as that observed for non-G4 DNA substrates. Thus, hRev1 can promote fork progression by either dislodging tetrad guanines to unfold the G4 DNA, which could assist in extension by other DNA polymerases, or hRev1 can prevent refolding of G4 DNA structures. The hRev1 mechanism of action against G-quadruplexes helps explain why replication progress is impeded at G4 DNA sites in Rev1-deficient cells and illustrates another unique feature of this enzyme with important implications for genome maintenance.

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Apr 25, 1991·Nature·A M ZahlerD M Prescott
Nov 1, 1988·Molecular and Cellular Biology·M H Linskens, J A Huberman
Aug 22, 1996·Nature·J R NelsonD C Hinkle
Oct 9, 1998·The Journal of Biological Chemistry·H SunN Maizels
Jul 4, 2001·Nucleic Acids Research·P MohagheghI D Hickson
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·C SchaffitzelA Plückthun
Feb 19, 2002·The Journal of Biological Chemistry·Lajos HaracskaLouise Prakash
Sep 18, 2002·Nucleic Acids Research·Michael D HuberNancy Maizels
Jul 15, 2004·Journal of the American Chemical Society·Anh Tuân PhanDinshaw J Patel
May 26, 2005·Nucleic Acids Research·Julian L Huppert, Shankar Balasubramanian
Sep 6, 2005·Nature Structural & Molecular Biology·Katrin PaeschkeHans Joachim Lipps
Oct 1, 2005·Science·Deepak T NairAneel K Aggarwal
Mar 15, 2006·Journal of Molecular Biology·Michael D HuberNancy Maizels
Jun 29, 2006·The Journal of Biological Chemistry·Yuji Masuda, Kenji Kamiya
Sep 16, 2006·Nucleic Acids Research·Tonatiuh Romero SalasCarole Saintomé
Oct 31, 2007·Methods : a Companion to Methods in Enzymology·Phillip A Rachwal, Keith R Fox
Jun 10, 2008·Chemical Communications : Chem Comm·Pravin S ShirudeShankar Balasubramanian
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Lauren S WatersGraham C Walker
May 26, 2009·Journal of Molecular Biology·Michael K SwanAneel K Aggarwal
Jul 11, 2009·Trends in Cell Biology·Hans J Lipps, Daniela Rhodes
Dec 15, 2010·Molecular Cell·Peter SarkiesJulian E Sale
Jul 21, 2011·Journal of Nucleic Acids·Aishwarya PrakashGloria E O Borgstahl
Jul 21, 2011·Journal of Nucleic Acids·Aishwarya PrakashGloria E O Borgstahl
Oct 25, 2011·Nucleic Acids Research·Peter SarkiesJulian E Sale
Feb 22, 2012·Methods : a Companion to Methods in Enzymology·Marco Di AntonioShankar Balasubramanian
Mar 28, 2012·Methods : a Companion to Methods in Enzymology·Michaela VorlíčkováJaroslav Kypr
Apr 17, 2012·The Journal of Physical Chemistry. B·Mohammad H QureshiHamza Balci
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Oct 4, 2012·Nature Reviews. Genetics·Matthew L BochmanVirginia A Zakian
Nov 2, 2012·Journal of the American Chemical Society·Amy Y Q Zhang, Shankar Balasubramanian
Feb 21, 2013·Nature Chemistry·Giulia BiffiShankar Balasubramanian
Apr 19, 2013·The Journal of Biological Chemistry·Ramanagouda Ramanagoudr-BhojappaKevin D Raney
May 10, 2013·Nature·Katrin PaeschkeVirginia A Zakian

❮ Previous
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Citations

Feb 18, 2016·Nucleic Acids Research·Oscar MendozaJean-Louis Mergny
Jul 16, 2014·International Journal of Molecular Sciences·Sabrina Harteis, Sabine Schneider
Aug 5, 2014·Expert Review of Clinical Pharmacology·Sebastian Müller, Raphaël Rodriguez
Dec 3, 2015·Molecular Cell·Davide SchiavoneAidan J Doherty
Jul 3, 2015·Frontiers in Genetics·Valentina CeaSimone Sabbioneda
Jun 28, 2016·Nucleic Acids Research·Colin G Wu, Maria Spies
Jul 5, 2016·Genes·Sanjay Kumar BhartiRobert M Brosh
Apr 19, 2017·The Journal of Biological Chemistry·Wezley C GriffinKevin D Raney
Feb 1, 2019·Genes·Leticia Koch Lerner, Julian E Sale
Nov 28, 2018·The EMBO Journal·Saša ŠvikovićJulian E Sale
Sep 6, 2014·The EMBO Journal·Davide SchiavoneJulian E Sale
Jul 8, 2015·EMBO Reports·Nancy Maizels
Mar 20, 2020·Human Molecular Genetics·Thomas J ButlerRobert M Brosh
Aug 5, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Theresa ZachejaPeter Burkovics
Jan 16, 2015·The Journal of Biological Chemistry·Alicia K Byrd, Kevin D Raney
Nov 19, 2017·Current Medicinal Chemistry·Katrina N EstepRobert M Brosh
Sep 25, 2017·Biochemical Society Transactions·Markus Sauer, Katrin Paeschke
Oct 24, 2018·International Journal of Molecular Sciences·Wei-Chung Tsao, Kristin A Eckert
Aug 21, 2019·Critical Reviews in Biochemistry and Molecular Biology·David Gallo, Grant W Brown
Feb 27, 2020·International Journal of Molecular Sciences·Antonio MaffiaSimone Sabbioneda
Oct 30, 2020·Frontiers in Cellular and Infection Microbiology·Ana PovedaVinicio Armijos-Jaramillo
Feb 18, 2021·Cell Chemical Biology·Pauline LejaultDavid Monchaud
Aug 26, 2017·Chemical Research in Toxicology·Maroof K Zafar, Robert L Eoff
Sep 8, 2017·Chemical Research in Toxicology·Michael A TrakselisAurea M Chu
Aug 31, 2021·Cellular and Molecular Life Sciences : CMLS·Fang-Yuan TengYong Xu
Nov 28, 2021·International Journal of Molecular Sciences·Rebecca LinkeKatrin Paeschke

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Methods Mentioned

BETA
footprinting
electrophoresis
circular dichroism
Fluorescence
fluorescence assay

Software Mentioned

Prism
GraphPad
ImageJ

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