Human RGS6 gene structure, complex alternative splicing, and role of N terminus and G protein gamma-subunit-like (GGL) domain in subcellular localization of RGS6 splice variants

The Journal of Biological Chemistry
Tapan K ChatterjeeRory A Fisher

Abstract

RGS proteins are defined by the presence of a semiconserved RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. RGS6 is a member of a subfamily of RGS proteins distinguished by the presence of DEP and GGL domains, the latter a G beta 5-interacting domain. Here we report identification of 36 distinct transcripts of human RGS6 that arise by unusually complex processing of the RGS6 gene, which spans 630 kilobase pairs of genomic DNA in human chromosome 14 and is interrupted by 19 introns. These transcripts arise by use of two alternative transcription sites and complex alternative splicing mechanisms and encode proteins with long or short N-terminal domains, complete or incomplete GGL domains, 7 distinct C-terminal domains and a common internal domain where the RGS domain is found. The role of structural diversity in the N-terminal and GGL domains of RGS6 splice variants in their interaction with G beta 5 and subcellular localization and of G beta 5 on RGS6 protein localization was examined in COS-7 cells expressing various RGS6 splice variant proteins. RGS6 splice variants with complete GGL domains interacted with G beta 5, irrespective of the type of N-terminal domain, while ...Continue Reading

References

Jan 1, 1986·Annual Review of Biochemistry·R A PadgettP A Sharp
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·J R HeplerT Kozasa
Jan 27, 1998·The Journal of Biological Chemistry·D M Berman, A G Gilman
Feb 12, 1998·Neuron·W HeT G Wensel
Sep 10, 1998·Biochemical and Biophysical Research Communications·J L CabreraV Z Slepak
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·B E SnowD P Siderovski
Dec 18, 1998·The Journal of Biological Chemistry·W ZengT M Wilkie
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·E R MakinoV Y Arshavsky
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·K LevayV Z Slepak
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·B E SnowD P Siderovski
Apr 6, 2000·The Journal of Biological Chemistry·N O DulinM J Dunn
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·L De VriesM G Farquhar
Jun 28, 2000·The Journal of Biological Chemistry·T K Chatterjee, R A Fisher
Jan 21, 2001·The Journal of Biological Chemistry·J H ZhangW F Simonds
Mar 30, 2001·The Journal of Biological Chemistry·S P HeximerK J Blumer
Jul 10, 2001·The Journal of Biological Chemistry·P G BurgonP J Casey

❮ Previous
Next ❯

Citations

Jun 13, 2009·Cell Biochemistry and Biophysics·Garret R AndersonKirill A Martemyanov
Jul 28, 2005·Science's STKE : Signal Transduction Knowledge Environment·John R Hepler
Aug 1, 2006·Journal of Biomedicine & Biotechnology·Kert MätlikMart Speek
Sep 25, 2010·Circulation Research·Jianqi YangRory A Fisher
Jun 6, 2013·Future Medicinal Chemistry·Christopher R BodleDavid L Roman
Mar 24, 2016·The AAPS Journal·Katelin E AhlersRory A Fisher
Nov 4, 2008·Cellular & Molecular Biology Letters·Jillian H HurstShelley B Hooks
Jul 26, 2005·Experimental Cell Research·Sang-Wook KangBon-Hong Min
May 12, 2006·Seminars in Cell & Developmental Biology·Gary B Willars
Jan 22, 2004·The Journal of Biological Chemistry·Zhengyu Liu, Rory A Fisher
Mar 5, 2005·Circulation Research·Evan L RiddlePaul A Insel
May 30, 2012·Journal of Neurochemistry·Evangelos LiapisVladlen Z Slepak
Jun 29, 2007·Molecular Pharmacology·Evan A YostCatherine H Berlot
Sep 5, 2020·Scientific Reports·Gwo-Tsann ChuangYi-Cheng Chang
Jun 5, 2013·The Journal of Biological Chemistry·Guillaume Bastin, Scott P Heximer
Feb 6, 2020·Molecular Pharmacology·Katelin E Ahlers-DannenRory A Fisher
Sep 18, 2004·Cancer Research·David M BermanXifeng Wu
Oct 3, 2020·Cell·Ikuo MasuhoKirill A Martemyanov
Jul 15, 2006·Cellular Signalling·Maria Abramow-NewerlyPeter Chidiac

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.