PMID: 7545920May 1, 1994Paper

Human rheumatoid factors with restrictive specificity for rabbit immunoglobulin G: auto- and multi-reactivity, diverse VH gene segment usage and preferential usage of V lambda IIIb

The Journal of Experimental Medicine
Q FangS M Fu

Abstract

To determine the molecular and functional properties of human rheumatoid factors (RF), we established stable hybridomas and Epstein-Barr virus-transformed B cell lines from the synovial fluid or peripheral blood of three patients with rheumatoid arthritis and one patient with systemic lupus erythematosus. 17 cell lines were obtained that produced high-titer immunoglobulin M (IgM) RF that reacted exclusively with rabbit but not human IgG or IgG of other mammalian species. Certain anti-rabbit IgG RF also had specificity for other mammalian antigens (Ag), including cytoskeletal proteins and intracellular proteins found in HeLa cells, as well as for Ag present in an extract prepared from the cell wall of group A streptococci. 13 of the 17 RF contained lambda-type light (L) chains, of which 12 were classified serologically as members of the lambda-L chain variable region (V lambda) subgroup, designated V lambda III. The heavy chain V region (VH) and V lambda sequences of nine of these IgM lambda RF were determined at the cDNA level. Five VH genes in three VH families were used by these antibodies (Ab), including VH1 (dp21/1-4b and dp10 [51p1]/hv1051), VH3 (dp38/3-15 and dp77/13-21), and VH4 (dp70/4-4b). The deduced V gene-encoded am...Continue Reading

References

Sep 1, 1976·Nucleic Acids Research·N Blin, D W Stafford
Aug 1, 1992·Immunological Reviews·A K StewartR S Schwartz
Aug 1, 1992·Immunological Reviews·T N MarionR J Hill
Jul 25, 1991·Nucleic Acids Research·R H DonJ S Mattick
Jun 1, 1990·Arthritis and Rheumatism·G S Firestein, N J Zvaifler
May 3, 1990·The New England Journal of Medicine·E D Harris
Jun 20, 1987·Journal of Molecular Biology·K H LeeT Honjo
Nov 6, 1987·Science·H W SchroederR M Perlmutter
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Apr 1, 1993·Scandinavian Journal of Immunology·P M YangP P Chen
Feb 1, 1959·Arthritis and Rheumatism·A PELTIER, C L CHRISTIAN
Mar 1, 1962·The Journal of Clinical Investigation·R C WILLIAMS, H G KUNKEL

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Citations

Nov 17, 2001·Arthritis and Rheumatism·S KaschnerT Dörner
Jan 5, 2002·Arthritis and Rheumatism·T Dörner, P E Lipsky
Feb 23, 1999·British Journal of Haematology·Y Y YangP P Chen
Jul 2, 1998·Clinical and Experimental Immunology·S C HuangE C Milner
Mar 1, 1995·The Journal of Experimental Medicine·C GiachinoA Lanzavecchia
Oct 1, 1995·Scandinavian Journal of Immunology·K AnderssonU Alkner
May 3, 2008·Leukemia & Lymphoma·Emma Flordal Thelander, Richard Rosenquist
Feb 23, 2010·Veterinary Immunology and Immunopathology·Yfke PasmanAzad K Kaushik
Dec 18, 2013·Veterinary Immunology and Immunopathology·Hsiao-Wei ChenKristy L Richards
Feb 11, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Willem J J FalkenburgTheo Rispens
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rahul PalShu Man Fu
Jul 1, 1995·Clinical and Diagnostic Laboratory Immunology·A Solomon, D T Weiss

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