PMID: 9420270Jan 7, 1998Paper

Human rhinovirus type 14:human immunodeficiency virus type 1 (HIV-1) V3 loop chimeras from a combinatorial library induce potent neutralizing antibody responses against HIV-1

Journal of Virology
A D SmithGail Ferstandig Arnold

Abstract

In an effort to develop a useful AIDS vaccine or vaccine component, we have generated a combinatorial library of chimeric viruses in which the sequence IGPGRAFYTTKN from the V3 loop of the MN strain of human immunodeficiency virus type 1 (HIV-1) is displayed in many conformations on the surface of human rhinovirus 14 (HRV14). The V3 loop sequence was inserted into a naturally immunogenic site of the cold-causing HRV14, bridged by linkers consisting of zero to three randomized amino acids on each side. The library of chimeric viruses obtained was subjected to a variety of immunoselection schemes to isolate viruses that provided the most useful presentations of the V3 loop sequence for potential use in a vaccine against HIV. The utility of the presentations was assessed by measures of antigenicity and immunogenicity. Most of the immunoselected chimeras examined were potently neutralized by each of the four different monoclonal anti-V3 loop antibodies tested. Seven of eight chimeric viruses were able to elicit neutralizing antibody responses in guinea pigs against the MN and ALA-1 strains of HIV-1. Three of the chimeras elicited HIV neutralization titers that exceeded those of all but a small number of previously described HIV imm...Continue Reading

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Citations

Apr 7, 2007·AIDS Research and Human Retroviruses·Timothy CardozoSusan Zolla-Pazner
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·P A KratzM Nassal
Jun 20, 2001·The Journal of General Virology·D van MeertenR M Verhaert
Mar 10, 2007·Angewandte Chemie·Martin Fischlechner, Edwin Donath
Sep 25, 2002·Clinics in Laboratory Medicine·James P McGettiganMatthias J Schnell

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