Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces

Frontiers in Bioengineering and Biotechnology
Wei SunGang Wu

Abstract

Promoting cell spreading is crucial to enhance bone healing and implant osteointegration. In this study, we investigated the stimulatory effect of human salivary histatin-1 (Hst-1) on the spreading of osteogenic cells in vitro as well as the potential signaling pathways involved. Osteogenic cells were seeded on bio-inert glass slides with or without the presence of Hst1 in dose-dependent or time-course assays. 1 scrambled and 6 truncated Hst1 variants were also evaluated. Cell spreading was analyzed using a well-established point-counting method. Fluorescent microscopy was adopted to examine the cellular uptake of fluorescently labeled Hst1 (F-Hst1) and also the cell spreading on sandblasted and acid etched titanium surfaces. Signaling inhibitors, such as U0126, SB203580, and pertussis toxin (PTx) were used to identify the potential role of extracellular-signal-regulated kinase, p38 and G protein-coupled receptor pathways, respectively. After 60 min incubation, Hst1 significantly promoted the spreading of osteogenic cells with an optimal concentration of 10 μM, while truncated and scrambled Hst1 did not. F-Hst1 was taken up and localized in the vicinity of the nuclei. U0126 and SB2030580, but not PTx, inhibited the effect of Hs...Continue Reading

References

Aug 1, 1991·Infection and Immunity·T XuF G Oppenheim
Sep 1, 1987·Journal of Microscopy·H J Gundersen, E B Jensen
Oct 1, 1995·The Biochemical Journal·Q Yan, A Bennick
Nov 22, 2001·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·T Albrektsson, C Johansson
Nov 11, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J GuicheuxJ Caverzasio
Mar 3, 2004·Biophysical Journal·Benjamin J Dubin-ThalerMichael P Sheetz
Mar 16, 2004·Cell·Grégory GiannoneMichael P Sheetz
Mar 18, 2004·The Journal of Pharmacy and Pharmacology·Kevin Kavanagh, Susan Dowd
Dec 20, 2005·Expert Review of Medical Devices·Cato LaurencinSaadiq F El-Amin
Mar 28, 2006·Journal of Cellular and Molecular Medicine·U KneserR E Horch
Mar 6, 2007·Journal of Periodontology·Bradley S McAllister, Kamran Haghighat
May 16, 2007·Current Biology : CB·James L McGrath
Sep 1, 2007·The Journal of the American Academy of Orthopaedic Surgeons·David J Hak
Jul 25, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Menno J OudhoffEnno C I Veerman
Nov 20, 2008·Journal of Biomaterials Science. Polymer Edition·Jun-Jay LeeSheng-Wen Hsiao
Aug 5, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Menno J OudhoffEnno C I Veerman
Sep 22, 2009·Journal of Dental Research·M J OudhoffE C I Veerman
Apr 29, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jan G M BolscherEnno C I Veerman
Feb 14, 2012·Journal of Clinical Periodontology·Kathrin DuskeThomas Kocher
Mar 17, 2012·Cytokine & Growth Factor Reviews·Jing Guo, Gang Wu
Apr 17, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Qinyi LiuTheresa Freeman
Apr 16, 2013·BioMed Research International·Seung-Ki MinIn-Sung Yeo
Aug 16, 2013·Journal of Pharmacy & Bioallied Sciences·Prasanna KumarGhousia Fathima
Dec 4, 2014·Biophysical Journal·Haguy WolfensonMichael P Sheetz
Apr 24, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Irene A van DijkJan Stap
Sep 29, 2015·Oral and Maxillofacial Surgery Clinics of North America·Tara AghalooSotirios Tetradis
Jul 13, 2016·BMC Medicine·Ippokratis PountosPeter V Giannoudis
Dec 13, 2016·Journal of Dental Research·I A van DijkJ Stap
May 20, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Irene A van DijkJan Stap
May 26, 2017·PloS One·Dhara ShahVinay Kumar Aakalu
Jul 26, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhidi WuXiaofeng Zhu
Jul 29, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pedro TorresVicente A Torres
Nov 23, 2017·Frontiers in Bioengineering and Biotechnology·Andrea GrossoNunzia Di Maggio
Dec 15, 2017·The International Journal of Periodontics & Restorative Dentistry·Chiara GiannasiGiulio Rasperini
Dec 10, 2019·Trends in Biochemical Sciences·Ossama Moujaber, Ursula Stochaj

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Methods Mentioned

BETA
GTPase
nucleotide exchange

Software Mentioned

Graphpad Prism
Graphpad

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