Human Saphenous Vein Response to Trans-wall Oxygen Gradients in a Novel Ex Vivo Conditioning Platform

Annals of Biomedical Engineering
Marco PiolaMonica Soncini

Abstract

Autologous saphenous veins are commonly used for the coronary artery bypass grafting even if they are liable to progressive patency reduction, known as 'vein graft disease'. Although several cellular and molecular causes for vein graft disease have been identified using in vivo models, the metabolic cues induced by sudden interruption of vasa vasorum blood supply have remained unexplored. In the present manuscript, we describe the design of an ex vivo culture system allowing the generation of an oxygen gradient between the luminal and the adventitial sides of the vein. This system featured a separation between the inner and the outer vessel culture circuits, and integrated a purpose-developed de-oxygenator module enabling the trans-wall oxygen distribution (high oxygen level at luminal side and low oxygen level at the adventitial side) existing in arterialized veins. Compared with standard cultures the bypass-specific conditions determined a significant increase in the proliferation of cells around adventitial vasa vasorum and an elevation in the length density of small and large caliber vasa vasorum. These results suggest, for the first time, a cause-effect relationship between the vein adventitial hypoxia and a neo-vasculariz...Continue Reading

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Citations

May 5, 2018·Tissue Engineering. Part a·Hannah A StrobelMarsha W Rolle
Oct 19, 2016·Annals of Biomedical Engineering·Marco PiolaGianfranco Beniamino Fiore
Mar 30, 2018·Frontiers in Cardiovascular Medicine·Matthijs Steven Ruiter, Maurizio Pesce
Jul 27, 2017·Cardiovascular Engineering and Technology·Marco Franzoni, Michael T Walsh
Aug 6, 2018·Journal of Cell Communication and Signaling·Andrzej Loesch, Michael R Dashwood

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