Human semen refrigeration at + 4 degrees C: bio-kinetic characteristics

Cell and Tissue Banking
Franco DonderoFernando Mazzilli

Abstract

The aim of our study was to evaluate the bio-kinetic characteristics of human semen refrigerated for different periods and to compare the effects of refrigeration at +4 degrees C against cryopreservation of human sperm at -196 degrees C. Semen was obtained from 30 male partners of infertile couples (infertile subjects) with the following semen profile: sperm count >or=10 x 10(6)/ml; progressive motility >or=20%; atypical forms <70% and white blood cells <1.0 x 10(6)/ml. Fifteen normospermic subjects were also selected as controls (control subjects). The following tests were carried out on basal, refrigerated and cryopreserved sperm: a) sperm kinetic properties (by Superimposed Image Analysis System); b) the Hypoosmotic Viability Test (HVT) (combined Hypoosmotic Swelling and Viability Test). The results of the study showed that the percentage recovery of kinetic properties and of HVT were optimum for up to 48 h. After refrigeration for 72 h, a drastic decrease in straight motility recovery was observed. No significant differences were observed between cryopreservation and refrigeration at +4 degrees C for 48 h for motility or HVT recoveries in samples from control subjects. However, in infertile subjects, a significant decrease ...Continue Reading

References

Oct 1, 1992·Fertility and Sterility·P J ChanB C Su
Dec 1, 1974·Clinical Obstetrics and Gynecology·W W Beck
Feb 1, 1981·Fertility and Sterility·D G Jaskey, M R Cohen
Oct 1, 1993·Fertility and Sterility·C SawetawanG S Prins

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Citations

Apr 21, 2012·Animal Reproduction Science·Sirirak BuarpungMongkol Techakumphu
Feb 20, 2010·Veterinary Radiology & Ultrasound : the Official Journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association·Géraldine BolenValeria Busoni
Jul 6, 2014·Zygote : the Biology of Gametes and Early Embryos·Sirirak BuarpungMongkol Techakumphu

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