Human serum albumin templated MnO2 nanosheets as an efficient biomimetic oxidase for biomolecule sensing.

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Jia GeRen Cai

Abstract

Herein, we have proposed a colorimetric biosensor for detection of acid phosphatase based on human serum albumin (HSA) templated MnO2 nanosheets (HSA-MnO2 NSs). HSA-MnO2 NSs as an efficient biomimetic oxidase could catalyze the oxidization of 3,3',5,5'-tetramethylbenzidine (TMB) to the coloured oxidation product (oxTMB). Acid phosphatase (ACP) could hydrolyze l-ascorbic acid-2-phosphate (AAP) to produce ascorbic acid, and ascorbic acid could lead to the decomposition of MnO2 NSs to Mn2+ ions, inhibiting the production of oxTMB. On the basis of this, we have demonstrated a novel colorimetric approach for the detection of acid phosphatase with the linear range from 50 μU mL-1 to 1500 μU mL-1 and a detection limit of 40 μU mL-1. The MnO2 NS-based colorimetric method has been successfully used to determine the content of acid phosphatase in real samples with satisfactory results.

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Citations

Aug 20, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Muhan JiangBaojiu Chen

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Methods Mentioned

BETA
atomic force microscopy
X-ray
AFM
Assay

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