Human serum alters cell culture behavior and improves spheroid formation in comparison to fetal bovine serum

Experimental Cell Research
Julia I HegerAndré Schmidt

Abstract

The use of fetal bovine serum (FBS) as growth supplement for human cell and tissue culture is widely spread in basic research as well as in clinical approaches, although several limitations must be considered, such as unstable composition and availability, biosafety and ethical aspects. Regarding interspecies differences, xenogeneic growth factors may evoke incompatibilities and non-desired interactions with human cells resulting in imprecise outcome of human-relevant data. In this study the functionality of human serum (HS) has been investigated in comparison to FBS by assessing proliferation, migration and invasion of the human cervical cancer cell lines SiHa and HeLa. The effects of both sera on spheroid formation were analyzed microscopically. Both, FBS and HS, stimulate cell proliferation and migration similarly, whereas HS significantly enhanced cell invasion. The spheroid formation assay revealed remarkable differences between both sera, especially for SiHa cells. While in FBS supplemented medium cells only formed loose aggregates, HS induced regularly shaped spheroids under all tested conditions. We were able to demonstrate that HS and FBS differently influence behavior of cells in culture which may have an impact on ex...Continue Reading

Citations

Dec 22, 2019·Biomolecules·Rachit BansalAnwar Sunna
May 1, 2019·Advanced Biosystems·Kasper RenggliAndreas Hierlemann
Oct 13, 2020·Advanced Healthcare Materials·Bryan D JamesJosephine B Allen
Dec 20, 2020·International Journal of Molecular Sciences·Filipa Quintela VieiraRegina Augusta Silva
Dec 29, 2020·Frontiers in Chemistry·Alexander H Jesmer, Ryan G Wylie
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Chrysanthi-Maria MoysidouRóisín Meabh Owens
Mar 18, 2021·Stem Cells International·Pavla TonarovaMarie Hubalek Kalbacova
Jul 31, 2021·Frontiers in Bioengineering and Biotechnology·Aranzazu VillasanteGordana Vunjak-Novakovic

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