Human serum proteins preseparated by electrophoresis or chromatography followed by tandem mass spectrometry

Journal of Proteome Research
John MarshallGeorge Jackowski

Abstract

Electrophoretic and chromatographic sample preparations were compared and together detected the presence of some 600 types of protein products in human serum. Proteins from crude serum preseparated by ionic electrophoresis, chromatography, or a combination of both were analyzed. Proteins were digested with trypsin or chymotrypsin. Naturally occurring peptides were also collected by reversed-phase chromatography. The resulting peptides were identified by tandem mass spectrometry. The peptides were either desorbed by a laser from a metal chip into a quadrupole-time-of-flight mass spectrometer or ionized as an electro-spray from reversed-phase chromatography via a metal needle under voltage into an ion-trap mass spectrometer. All of the commonly known proteins associated with serum were detected, and the two mass spectrometers agreed on the identity of abundant serum proteins. Preseparation of serum proteins prior to digestion markedly enhanced the capacity to detect un-common proteins from blood. Electrophoretic- and chromatography-based experiments were found to be complementary. Many novel cellular proteins not previously associated with serum were recorded.

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Citations

May 13, 2010·Clinical Proteomics·Gitika PanickerElizabeth R Unger
Aug 6, 2008·Analytical Chemistry·James A DowellLingjun Li
Aug 18, 2009·Expert Review of Proteomics·Hari R KumarJohn A Sandoval
Apr 3, 2012·Expert Review of Proteomics·Feng YangRichard D Smith
Feb 23, 2010·Journal of Proteomics·Peter BowdenJohn G Marshall
Nov 24, 2007·Journal of Pharmaceutical and Biomedical Analysis·Antonella ArestaAngelo Paradiso
May 26, 2005·Rapid Communications in Mass Spectrometry : RCM·Anne K CallesenOle N Jensen
Sep 1, 2011·Mass Spectrometry Reviews·Peihong ZhuJohn G Marshall
Jul 10, 2007·Analytical Biochemistry·Ziad J SahabQing-Xiang Amy Sang
Sep 1, 2014·Toxicon : Official Journal of the International Society on Toxinology·Jan MatysiakZenon J Kokot
Dec 19, 2014·Analytical and Bioanalytical Chemistry·Angelique Florentinus-MefailoskiJohn G Marshall
Nov 9, 2004·Journal of Mass Spectrometry : JMS
Nov 3, 2017·Clinical Proteomics·Jaimie DufresneJohn Marshall
Jan 1, 2020·Clinical Proteomics·Jaimie DufresneJohn G Marshall
Jul 9, 2020·Clinical Proteomics·Thanusi ThavarajahJohn G Marshall
Jan 2, 2019·Clinical Proteomics·Jaimie DufresneJohn G Marshall
Dec 7, 2018·Clinical Proteomics·Jaimie DufresneJohn G Marshall
Feb 28, 2018·Analytical Biochemistry·Jaimie DufresneJohn G Marshall
Apr 10, 2021·Scientific Reports·Steven LynhamRichard Beatson
Mar 7, 2009·Journal of Proteome Research·Monika TucholskaJohn Marshall
Jun 30, 2021·Clinical Proteomics·Angelique Florentinus-MefailoskiJohn G Marshall

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