Human skeletal muscle lactate dehydrogenase activity in the presence of some alcohol dehydrogenase inhibitors

Basic & Clinical Pharmacology & Toxicology
Jaroslaw DudkaElzbieta Korobowicz

Abstract

Methanol, ethylene glycol and other alcohol intoxications are complicated by severe acidosis which could be caused by formation of metabolic acids and additionally lactic acid production. An increasing nicotinamide adenine dinucleotide reduced/nicotinamide adenine dinucleotide oxidized (NADH/NAD) ratio during alcohol biotransformation is responsible for the induction of lactic acidosis. The main purpose of the present paper was to evaluate the effect of 4-methylpyrazole, cimetidine, ethylenediaminetetraacetic acid disodium salt, ethanol and methanol on lactate dehydrogenase (E.C. 1.1.1.27) activity and to discuss this issue. The activity of the enzyme was determined spectrophotometrically, in vitro using human enzyme skeletal muscle homogenates. 4-Methylpyrazole, cimetidine and ethylenediaminetetraacetic acid disodium salt at concentrations 0.01, 0.1, 1.0 mM and 12.5, 25.0, 50.0 mM of ethanol and methanol were studied. Our results showed that cimetidine increased lactate dehydrogenase activity as compared to the control at all tested concentrations. Such activity was noted for 4-methylpyrazole at 0.1 mM and higher concentration. By contrast, no significant effect on lactate dehydrogenase activity in the presence of ethylenediam...Continue Reading

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Citations

Feb 18, 2010·Progress in Nuclear Magnetic Resonance Spectroscopy·Su Xu, Jun Shen
Jan 30, 2007·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Su XuJun Shen
Jan 26, 2016·Basic & Clinical Pharmacology & Toxicology·Sergey ZakharovDaniela Pelclova
Dec 23, 2009·Human & Experimental Toxicology·Amal A El-BakaryHala A Malek
Oct 7, 2005·Journal of Applied Toxicology : JAT·Jaroslaw DudkaElzbieta Korobowicz

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