Human skin lymph derived from irritant and allergic contact dermatitis: interleukin 10 is increased selectively in elicitation reactions

Dermatology : International Journal for Clinical and Investigative Dermatology
C U BrandL R Braathen

Abstract

Recent reports suggested an immunomodulatory role for interleukin 10 (IL-10) in contact hypersensitivity. To investigate if IL-10 is important in the regulation of irritant and allergic contact dermatitis (CD), IL-10 and interferon gamma (IFN-gamma) protein levels were measured in normal skin lymph, in lymph derived from irritant and allergic (primary sensitization and elicitation) CD and in skin blister fluid from an elicitation reaction. A superficial lymph vessel was cannulated microsurgically on the lower leg of 18 healthy volunteers. Lymph was collected twice daily. Protein levels of IL-10 and IFN-gamma were determined using commercially available ELISA kits and messenger RNA was estimated by a reverse-transcriptase polymerase chain reaction (PCR) method. Whereas the IL-10 levels in lymph derived from irritant CD and primary sensitization of allergic CD, similarly to those obtained from normal untreated skin, remained below 4.4 pg/ml, the IL-10 levels increased manifold both in the primary allergic reaction (928.5 pg/ml) and the elicitation of allergic CD (124 pg/ml). The levels of IFN-gamma also increased in all volunteers exhibiting an eczematous skin reaction and showed a tendency to be inversely correlated with IL-10. ...Continue Reading

Citations

Jul 1, 2011·Immunologic Research·Pawel KalinskiDavid L Bartlett
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