PMID: 8017Jun 21, 1976

Human skin proteases. Separation and characterization of two acid proteases resembling cathepsin B1 and cathepsin D and of an inhibitor of cathepsin B1

Archives of Dermatological Research
J E Fräki

Abstract

Two acid proteases, one hydrolysing hemoglobin and the other hydrolysing benzoyl arginine naphthyamide (BANA), were separated and partially purified from human skin buffer extract. The acid protease hydrolysing hemoglobin was purified about 190 fold by Sephadex G-100 gel filtration and DEAE-cellulose chromatography. It hydrolysed hemoglobin at pH 3.5, casein at pH 5.8 and skin protein substrate at pH 6.0. It did not markedly hydrolyse synthetic protease substrates. The molecular size of this protease was 38000. The protease was insensitive to common protease modifiers and closely resembles cathepsin D purified from other organs. The BANA-hydrolysing acid protease was purified about 760 fold by Sephadex G-100 gel filtration and affinity chromatography on organomercurial Sepharose 4B gel. It preferentially hydrolysed BAEE, BANA and BAA with an optimum at pH 5.8. The hydrolysis of BAPA, LeuNA and protein substrates was very low. This acid protease was found to be highly dependent on reducing agents, as DTT, and chelating agents, as EDTA, and was inhibited by pCMB and TLCK. The molecular size of the enzyme was 28000. This protease closely resembles cathepsin B1 purified from other organs. Human skin was also shown to contain a low ...Continue Reading

References

Sep 1, 1975·The Journal of Investigative Dermatology·J E HeikkinenC R Jansén
Jan 1, 1975·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·M Järvinen, V K Hopsu-Havu
Jan 1, 1975·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·M Järvinen, V K Hopsu-Havu
Sep 1, 1975·The Journal of Investigative Dermatology·G S LazarusN Levine
Apr 1, 1973·The Biochemical Journal·A J Barrett
Apr 1, 1973·The Biochemical Journal·P M Starkey, A J Barrett
Feb 1, 1974·The Journal of Investigative Dermatology·G S Lazarus, J T Dingle
Feb 1, 1974·The Biochemical Journal·M C BurleighG S Lazarus
Jan 1, 1972·Archiv Für Dermatologische Forschung·J E Fräki, V K Hopsu-Havu
Apr 1, 1974·The Journal of Investigative Dermatology·G S Lazarus
Feb 1, 1974·The Biochemical Journal·A A SwansonS S Spicer
Jan 1, 1972·Archiv Für Dermatologische Forschung·J E Fräki, V K Hopsu-Havu
Jan 1, 1971·Acta Chemica Scandinavica·J Suominen, V K Hopsu-Havu
Apr 1, 1970·The Biochemical Journal·A J Barrett
Feb 15, 1965·Biochimica Et Biophysica Acta·K UDAKA, H HAYASHI

Citations

Dec 1, 1979·Clinical and Experimental Dermatology·J E Fräki
Jun 1, 1979·Journal of Cutaneous Pathology·J E FräkiV K Hopsu-Havu
Jul 21, 1977·Archives of Dermatological Research·J E Fräki, V K Hopsu-Havu
Jan 1, 1986·Archives of Dermatological Research·J E FräkiG S Lazarus
Jun 1, 1981·The British Journal of Dermatology·V K Hopsu-HavuM Järvinen
Apr 1, 1981·International Journal of Dermatology·V K Hopsu-Havu, J E Fräki
Nov 6, 2001·Nature Immunology·B SchittekC Garbe
Nov 1, 1979·European Journal of Biochemistry·J F LenneyA G Lee
May 21, 2019·Journal of Biomolecular Structure & Dynamics·Fakhra AminBilqees Bano

Related Concepts

Benzoylarginine-2-Naphthylamide
Cathepsins
Liquid Chromatography
Eryhem
Hydrogen-Ion Concentration
Hydrolysis
Peptide Hydrolases
Skin

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