Human SNF5 arming of double-deleted vaccinia virus shows oncolytic and cytostatic activity against central nervous system atypical teratoid/rhabdoid tumor cells.

Cancer Gene Therapy
Satbir ThakurAru Narendran

Abstract

Central nervous system (CNS) atypical teratoid/rhabdoid tumor (AT/RT) is a rare, aggressive tumor that most often affects very young children. The common decisive molecular defect in AT/RT has been shown to be a single genetic alteration, i.e., the loss of hSNF5 gene that encodes for a subunit of the SWI/SNF complex that modulates chromatin remodeling activities. As a result, AT/RT cells display unregulated cell proliferation due to the dysfunction of an important epigenetic control. We have previously demonstrated the preclinical efficacy of the oncolytic double-deleted vaccinia virus (VVDD) against AT/RT. Here we report the establishment of a modified VVDD engineered to express wild type hSNF5 gene. We show that this reconstructed vaccinia virus retains comparable infectivity and in vitro cytotoxicity of the parent strain. However, in addition, hSNF5-arming of VVDD results in a decreased cell cycle S phase population and down-regulation of cyclin D1. These findings suggest that hSNF5-arming of VVDD may increase the efficacy in the treatment of AT/RT and validates, as a proof-of-concept, an experimental approach to enhance the effective use of novel modified oncolytic viruses in the treatment of tumors with loss of a tumor sup...Continue Reading

References

Jul 26, 2002·Molecular and Cellular Biology·Zhi-Kai ZhangGanjam V Kalpana
Nov 8, 2003·The Journal of Biological Chemistry·Igor OruetxebarriaC Peter Verrijzer
Jan 12, 2005·The Journal of Urology·Istvan FodorMichael Lilly
Nov 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Michael S IsakoffCharles W M Roberts
Sep 3, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·XueQing LunPeter A Forsyth
Sep 22, 2012·Pediatric Blood & Cancer·Mark W KieranJaclyn A Biegel
Nov 21, 2012·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Christian Frezza, Carla P Martins
Oct 9, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Herbert J ZehDavid L Bartlett
May 21, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Stephanie Downs-CannerDavid L Bartlett
Oct 4, 2016·Cancer Management and Research·Ahitagni BiswasPramod Kumar Julka
May 11, 2018·Journal of Korean Neurosurgical Society·Elizabeth Anne RichardsonAnnie Huang
Aug 25, 2019·Molecular Cancer·Miao PengZhaoyang Zeng
Dec 25, 2019·Current Opinion in Biotechnology·Erkko Ylösmäki, Vincenzo Cerullo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Epigenetics and Chromatin (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on chromatin and its role in cancer epigenetics please follow this feed to learn more.

Cancer Epigenetics Chromatin Complexes (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on chromatin complexes and their role in cancer epigenetics.

Related Papers

Molecular Therapy : the Journal of the American Society of Gene Therapy
Herbert J ZehDavid L Bartlett
Polish Journal of Pathology : Official Journal of the Polish Society of Pathologists
W Biernat
AJNR. American Journal of Neuroradiology
K K MoellerT M Moriarty
© 2021 Meta ULC. All rights reserved