Human sperm anatomy: ultrastructural localization of the cannabinoid1 receptor and a potential role of anandamide in sperm survival and acrosome reaction

The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology
Saveria AquilaAndò Sebastiano

Abstract

Recently, the endocannabinoid (EC) system and the presence of CB1 receptor (CB1-R), have been identified in human sperm. However, the effects of EC receptor ligands such as anandamide (N-arachidonoylethanolamine) and the role of EC system in male fertility is still largely unexplored. In the present study, we investigated the ultrastructural compartmentalization of CB1-R and analyzed the effects of its stimulation by using a stable analog of anandamide, 2-methylarachidonyl-2'-fluoro-ethylamide (MET-F-AEA). We focused particularly on sperm survival and acrosin activity. The study of human sperm anatomy by transmission electron microscopy with immunogold analysis revealed the location of the CB1-R prevalently in the sperm membranes of the head and interestingly on the mitochondria. The effect of different concentrations of MET-F-AEA from 100 nM to 1 microM evidenced a significant decrease of sperm survival. Interestingly, we analyzed this negative effect at molecular level, testing the EC action on different known sperm survival targets. MET-F-AEA-treatment decreased both pBCL2 and pAkt, two prosurvival proteins, and increased pPTEN expression which is the main regulator of the PI3K/Akt pathway. Moreover, a biphasic effect was ob...Continue Reading

References

May 1, 1989·Journal of Andrology·W P KennedyL J Zaneveld
Sep 1, 1987·Fertility and Sterility·C G Smith, R H Asch
Jan 1, 1985·Progress in Neuro-psychopharmacology & Biological Psychiatry·M O Maykut
Oct 1, 1983·Journal of Psychoactive Drugs·D J Powell, R W Fuller
Jan 1, 1995·Life Sciences·C Song, A C Howlett
Feb 24, 1995·The Journal of Biological Chemistry·D ShireP Ferrara
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·H SchuelS Zimmerman
Dec 1, 1993·Molecular Reproduction and Development·M C ChangH Schuel
May 2, 1997·The Journal of Biological Chemistry·T ItoW S May
Jul 15, 1998·Human Reproduction·L R Fraser
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·X WuC L Sawyers
Apr 6, 2000·The Biochemical Journal·T Gómez del PulgarM Guzmán
Apr 13, 2000·The European Journal of Neuroscience·A PorcellaL Pani
May 12, 2000·European Journal of Biochemistry·M MacCarroneA Finazzi-Agrò
Mar 30, 2001·The Journal of Biological Chemistry·B C PariaS K Dey
Jun 8, 2001·Biochemical and Biophysical Research Communications·T WengerI Gerendai
Jun 1, 2002·Science·Lewis C Cantley
Jul 11, 2002·The Journal of Clinical Endocrinology and Metabolism·Saveria AquilaSebastiano Andò
Aug 3, 2002·European Journal of Biochemistry·Isabel MatiasVincenzo Di Marzo
Sep 19, 2002·Molecular Reproduction and Development·Herbert SchuelAlexandros Makriyannis
Dec 31, 2002·Chemistry and Physics of Lipids·Herbert SchuelAndrea Giuffrida
Jan 21, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Theodore A SarafianMichael D Roth
Jul 23, 2003·Human Reproduction·Saveria AquilaSebastiano Andò
Aug 23, 2003·Cell Death and Differentiation·M Maccarrone, A Finazzi-Agró
Dec 6, 2003·Reproductive Biomedicine Online·Sergio OehningerStephen Beebe
Dec 20, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Boram ParkMichelle Glass
Mar 17, 2004·Fertility and Sterility·Uwe PaaschHans-Jürgen Glandera
Jun 24, 2004·Seminars in Cell & Developmental Biology·Ramon Parsons
Sep 2, 2004·Nature Reviews. Drug Discovery·Vincenzo Di MarzoLuciano De Petrocellis
Jan 22, 2005·Annals of the New York Academy of Sciences·Uwe PaaschHans-Juergen Glander
Mar 9, 2005·Cancer Research·Sami SarfarazHasan Mukhtar
Jun 10, 2005·The Journal of Clinical Endocrinology and Metabolism·Saveria AquilaSebastiano Andò

❮ Previous
Next ❯

Citations

Mar 6, 2012·Nature Neuroscience·Giovanni BénardGiovanni Marsicano
Aug 19, 2015·Journal of Assisted Reproduction and Genetics·Stefan S du PlessisArun Syriac
Nov 9, 2016·Frontiers in Physiology·Juan Mendizabal-ZubiagaPedro Grandes
Feb 12, 2014·Human Reproduction Update·Cinzia RapinoMauro Maccarrone
Sep 2, 2017·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Arnau Busquets-GarciaGiovanni Marsicano
Dec 26, 2018·Molecular Reproduction and Development·Eduardo Martínez-LeónEmilce Silvina Díaz
Jan 1, 2011·Journal of Basic and Clinical Physiology and Pharmacology·Tatiana Bregman, Ester Fride
Feb 16, 2019·Current Molecular Pharmacology·Lingyan YeNaiming Zhou
Feb 13, 2020·International Journal of Molecular Sciences·Rosaria MeccarielloRiccardo Pierantoni
Oct 20, 2015·Journal of Basic and Clinical Physiology and Pharmacology·Isiaka A AlagbonsiToyin M Salman
Jan 20, 2011·Expert Opinion on Therapeutic Targets·Anna Maria MalfitanoMaurizio Bifulco
May 28, 2011·British Journal of Pharmacology·Daniel J MorganKen Mackie
Nov 23, 2016·The Journal of Endocrinology·Shuai Li, Wipawee Winuthayanon
Jul 10, 2020·Nature·Daniel Jimenez-BlascoGiovanni Marsicano
Dec 10, 2020·BMC Nutrition·Abdullateef Isiaka Alagbonsi, Luqman Aribidesi Olayaki
Feb 1, 2020·Reproductive Biomedicine Online·France VerhaegheNathalie Rives
May 11, 2021·Reproductive Sciences·Bruno M Fonseca, Irene Rebelo
Aug 31, 2021·Reviews in Endocrine & Metabolic Disorders·Farah MeahMary Ann Emanuele
Oct 29, 2021·Current Opinion in Endocrinology, Diabetes, and Obesity·Kimberly S RyanJamie O Lo

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.