Human sperm chromosome analysis after microinjection into hamster oocytes

Journal of Assisted Reproduction and Genetics
J CozziB Sèle

Abstract

Subzonal sperm insemination (SUZI) into hamster oocytes was performed to establish the karyotypes of the fertilizing spermatozoa. Spermatozoa from two males with normal semen parameters were microinjected. Of 72 (52 + 20) analyzed sperm chromosome metaphases, only 1 (1.4%) was considered abnormal, showing a structural abnormality. No hyperhaploidy was observed. Rates of sperm chromosomal abnormalities after microinjection were not higher than those reported previously using zona-free egg insemination, suggesting that the SUZI procedure per se does not increase sperm chromosomal abnormalities. The use of subzonal insemination into hamster oocytes for the study of human sperm chromosomes in males with low sperm counts is discussed.

References

Jun 1, 1991·American Journal of Medical Genetics·R H MartinA Rademaker
Jan 1, 1991·Human Reproduction·J CohenH Liu
Mar 24, 1990·Lancet·S FishelC Versaci
Jan 1, 1990·Archives of Andrology·S C NgS S Ratnam
Oct 1, 1988·Lancet·S C NgV H Goh
Feb 1, 1989·Human Reproduction·T A BongsoS Ganatra
Sep 1, 1988·Journal of Reproduction and Fertility·R H MartinA Rademaker
May 1, 1993·Prenatal Diagnosis·J SelvaP Jouannet

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Citations

Jan 22, 1998·Mutation Research·D E McFadden, J M Friedman
Sep 3, 2003·Andrologia·A JungW-B Schill

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