Human sperm plasma membranes possess alpha-D-mannosidase activity but no galactosyltransferase activity

Biology of Reproduction
D R TulsianiM C Orgebin-Crist

Abstract

Previous studies from this laboratory and others have identified several enzymes on the surface of mammalian spermatozoa. Some of these enzymes, namely a galactosyltransferase and a novel alpha-D-mannosidase, are believed to play a ligand-like role in recognizing and binding to the complementary moiety(ies) present on zona pellucida glycoconjugates. However, little or no information is available about the occurrence of these enzymes in human spermatozoa. In the present report, we show that a very small amount of the total galactosyltransferase activity present in human semen is associated with spermatozoa. Moreover, our failure to find a significant amount of the enzyme on sperm plasma membranes suggests that the enzyme is not associated with the sperm surface. Therefore, it is unlikely that galactosyltransferase in humans has the same ligand-like role in zona binding that is demonstrated in mouse sperm. In contrast, nearly 5% of alpha-D-mannosidase activity was repeatedly found in the salt-washed plasma membrane fraction. The recovery and enrichment of the alpha-D-mannosidase was nearly one-half that observed for adenylate cyclase and nearly one-third that for phosphodiesterase I, the two sperm plasma membrane marker enzymes. ...Continue Reading

Citations

Dec 18, 2001·Molecular Reproduction and Development·W WeerachatyanukulN Tanphaichitr
Aug 1, 1992·Journal of Assisted Reproduction and Genetics·J Tesarik
Jul 1, 1994·Journal of Assisted Reproduction and Genetics·P F LevayF R Fourie
Apr 21, 2007·Science in China. Series C, Life Sciences·XueXiong SongYiBing Han
Dec 4, 2001·Journal of Reproductive Immunology·Masahiko MaegawaToshihiro Aono
Jul 12, 2002·Insect Biochemistry and Molecular Biology·F CattaneoM E Pasini
Jun 13, 2008·Journal de la Société de biologie·Catherine SerresPierre Jouannet
Jan 15, 2013·Human Reproduction·Gary F Clark
Mar 1, 1996·American Journal of Reproductive Immunology : AJRI·R J AitkenM van Duin
Nov 3, 2010·Asian Journal of Andrology·Andrew T ReidBrett Nixon
Jun 22, 2014·Biochemical and Biophysical Research Communications·Gary F Clark
Nov 12, 2002·Archives of Andrology·R GamzuH Yavetz
Sep 13, 2001·Molecular Reproduction and Development·M E PerottiJ H Hackstein
Jun 19, 2007·International Journal of Andrology·S L Perez MartinezP V Miranda
Jun 24, 2015·Insect Biochemistry and Molecular Biology·Jari IntraMaria Enrica Pasini
May 15, 2012·Theriogenology·C A RetamalM L López
Mar 25, 2015·Insect Biochemistry and Molecular Biology·Kimberly StephensRichard A Cardullo
Mar 28, 2006·Current Topics in Developmental Biology·Julian L Wong, Gary M Wessel
Dec 22, 1998·Biology of Reproduction·C R Loeser, D R Tulsiani
Jun 23, 2001·Molecular Human Reproduction·M RattanachaiyanontN Tanphaichitr
Feb 7, 2003·The Journal of Biological Chemistry·Philip C N ChiuWilliam S B Yeung
May 1, 1992·Archives of Andrology·L C García DíezJ M Miralles
Aug 25, 1999·Molecular Human Reproduction·J A AlhadeffB Bean
Jul 1, 1991·Archives of Andrology·M KamadaS S Koide
Jul 1, 1996·Molecular Reproduction and Development·M AvilésJ Ballesta
Feb 11, 1999·International Journal of Andrology·V SrikanthK Balasubramanian
Nov 1, 1995·Andrologia·E Töpfer-PetersenF Sinowatz
Jan 19, 2005·Reproduction in Domestic Animals = Zuchthygiene·G KaulB Singh
Sep 3, 2020·Journal of Proteome Research·Rongxia LanShisheng Sun
Aug 7, 2021·Frontiers in Cell and Developmental Biology·Ying WangPhilip C N Chiu
Jul 7, 1995·Science·R J Aitken

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