Human spermatozoa possess an IL4I1 l-amino acid oxidase with a potential role in sperm function

Reproduction : the Official Journal of the Society for the Study of Fertility
B HoustonR John Aitken

Abstract

Reactive oxygen species (ROS) are known to play an important role in the regulation of human sperm function. In this study, we demonstrate for the first time that human spermatozoa possess interleukin-induced gene 1 (IL4I1), an l-amino acid oxidase (LAAO) which is capable of generating ROS on exposure to aromatic amino acids in the presence of oxygen. The preferred substrates were found to be phenylalanine and tryptophan while the enzyme was located in the acrosomal region and midpiece of these cells. In contrast to equine and bovine spermatozoa, enzyme activity was lost as soon as the spermatozoa became non-viable. On a cell-to-cell basis human spermatozoa were also shown to generate lower levels of hydrogen peroxide than their equine counterparts on exposure to phenylalanine. Stimulation of LAAO activity resulted in the induction of several hallmarks of capacitation including tyrosine phosphorylation of the sperm flagellum and concomitant activation of phospho-SRC expression. In addition, stimulation of LAAO resulted in an increase in the levels of acrosomal exocytosis in both the presence and absence of progesterone stimulation, via mechanisms that could be significantly reversed by the presence of catalase. As is often the ...Continue Reading

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Citations

Mar 8, 2016·Animal Reproduction Science·J RocaH Rodriguez-Martinez
Jun 10, 2016·Human Reproduction Update·Elisabetta Tosti, Yves Ménézo
Sep 9, 2016·Cellular and Molecular Life Sciences : CMLS·Jacinta H MartinBrett Nixon
Jul 28, 2017·Molecular Reproduction and Development·Robert J Aitken
Jun 9, 2020·Andrologia·Damayanthi DurairajanayagamRalf Henkel
Jul 25, 2019·Cells·Valérie Molinier-FrenkelFlavia Castellano
Feb 1, 2015·Reproduction, Fertility, and Development·Robert John AitkenParviz Gharagozloo
Oct 27, 2020·Frontiers in Endocrinology·Robert John Aitken, Mark A Baker
Feb 17, 2021·Biology of Reproduction·Haolin ZhangKentaro Nagaoka
Jun 10, 2021·Applied Microbiology and Biotechnology·Kosuke KasaiTomisato Miura
Jul 29, 2021·Molecular Reproduction and Development·Gen L TakeiPablo E Visconti
Oct 30, 2021·Antioxidants & Redox Signaling·Steven Serafini, Cristian O'Flaherty
Nov 14, 2021·Journal of Animal Science and Biotechnology·Yentel Mateo-OteroMarc Yeste

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