Human sphingomyelin synthase 1 gene (SMS1): organization, multiple mRNA splice variants and expression in adult tissues

Gene
Alexandra V RozhkovaLyudmila V Dergunova

Abstract

We have previously characterized the structure of the human MOB gene (TMEM23), which encodes a hypothetical transmembrane protein (Vladychenskaya et al., 2002, 2004). The primary structure of the peptide that we predicted coincided completely with the amino acid sequence of the later identified sphingomyelin synthase 1 protein (SMS1), which catalyses the transfer of a phosphorylcholine moiety from phosphatidylcholine to ceramide, producing sphingomyelin and diacylglycerol (Huitema et al., 2004; Yamaoka et al., 2004). The gene we found was the SMS1 gene. The combination of in silico and RT-PCR data helped us identify and characterize numerous new transcripts of the human SMS1 gene. We identified mRNA isoforms that vary in the 5'-untranslated region (UTR) and encode the full-length protein, and transcripts resulting from alternative combinations of the exons in the coding region of the gene and the 3'-UTR. Comparison of the discovered transcripts' structures with the sequence of human chromosome 10 showed that the human SMS1 gene comprises at least 24 exons. RT-PCR and real-time PCR data showed that the expression patterns of the alternative SMS1 transcripts are tissue specific. Our results indicate that the regulation of SMS1 ex...Continue Reading

References

May 25, 1982·Biochemistry·D R Voelker, E P Kennedy
Apr 1, 1994·Trends in Biochemical Sciences·A P Geballe, D R Morris
Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
Jan 1, 1997·Protein Science : a Publication of the Protein Society·J SchultzP Bork
Nov 28, 1997·Archives of Dermatological Research·C C GeilenC E Orfanos
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·H ZhangJ C Reed
Apr 18, 2001·Biochemistry·Y A HannunK M Argraves
Oct 19, 2001·Biochemical and Biophysical Research Communications·E A GrzybowskaJ A Siedlecki
Dec 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Benjamin P LewisSteven E Brenner
Sep 5, 2003·The Journal of Biological Chemistry·Francisco N BarreraJosé L Neira
Dec 9, 2003·Trends in Biochemical Sciences·Chongwoo A Kim, James U Bowie
Dec 20, 2003·The EMBO Journal·Klazien HuitemaJoost C M Holthuis
Jun 15, 2004·Current Opinion in Structural Biology·Liana F LareauSteven E Brenner
Jul 31, 2004·Nature Genetics·Jill A HolbrookAndreas E Kulozik
Aug 19, 2004·Gene·Irina P VladychenskayaSvetlana A Limborska
May 4, 2005·Genome Research·Alexei A SharovMinoru S H Ko
Oct 29, 2005·Nucleic Acids Research·Monica CuccureseConcetta Pietropaolo
Apr 24, 2007·The Journal of Biological Chemistry·Fikadu Geta TafesseJoost C M Holthuis
Aug 13, 2008·Biochimica Et Biophysica Acta·Calvin YeangXian-Cheng Jiang
Feb 24, 2009·Biochemical and Biophysical Research Communications·Motohiro Tani, Osamu Kuge

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Citations

Apr 4, 2015·Molekuliarnaia biologiia·A V RozhkovaL V Dergunova
Oct 28, 2016·PloS One·Anke WittmannThomas Floss
Jan 3, 2018·Cell and Tissue Research·Ivan B FilippenkovLyudmila V Dergunova
Mar 14, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sitapriya MoorthiChiara Luberto
Apr 29, 2015·Molecular and Cellular Biochemistry·Olga Yu SudarkinaLyudmila V Dergunova
Jul 28, 2016·Neurogenetics·Ivan B FilippenkovLyudmila V Dergunova
Sep 26, 2013·Molecular Biology Reports·Lyudmila V DergunovaSvetlana A Limborska

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