Human stem cell factor promoter deoxyribonucleic acid sequence and regulation by cyclic 3',5'-adenosine monophosphate in a Sertoli cell line

Endocrinology
W E TaylorS Bhasin

Abstract

Stem cell factor (SCF) gene expression is regulated by FSH in testicular Sertoli cells. Many functions of FSH are mediated through the second messenger cAMP. We show that cAMP activates transcription of the human SCF promoter in a Sertoli cell line. The human SCF promoter was cloned in cosmid vector pWE15, and its DNA sequence was determined for the promoter region extending 2.3 kilobase pairs upstream from the translation start site at +184 bp. The in vivo messenger RNA (mRNA) start site, by primer-extension studies, was located in exon 1 at +109 bp in human testis mRNA, and at +99 bp in mouse SF7 Sertoli cell line or GC1 germ cell line mRNA. To test which regions of the SCF promoter are necessary for regulation by cAMP, a series of 5'-end deletions of this region were cloned onto the luciferase reporter gene in plasmid pXP1. The SCF promoter region was fused to luciferase downstream (at +120) from its +109 mRNA start site, extending upstream a variable distance to BstXI (-162), BamHI (-313), Bgl2 (-853), or XbaI (-2185). The shortest of these fragments extending only to -162 bp, contains possible SP1 and AP-2 elements. When mouse Sertoli SF7 or human JEG.3 cell lines were transfected with these plasmids, all of the mutants we...Continue Reading

Citations

Apr 6, 2002·British Journal of Pharmacology·Carla Alexandra Da SilvaNelly Frossard
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·N F Gonzalez-CadavidS Bhasin
Dec 10, 2003·British Journal of Pharmacology·Carla Alexandra Da SilvaNelly Frossard
Jun 18, 2005·Memórias do Instituto Oswaldo Cruz·Carla A Da Silva, Nelly Frossard
Sep 27, 2002·American Journal of Reproductive Immunology : AJRI·Paola GrimaldiRaffaele Geremia
Oct 18, 2012·Physiological Reviews·Johan Lennartsson, Lars Rönnstrand
Jun 5, 2020·Histochemistry and Cell Biology·Jia LiMeijia Zhang
Feb 5, 2004·Journal of Andrology·Mary A Bedell, Aparna Mahakali Zama

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