Human stem cell neuronal differentiation on silk-carbon nanotube composite.

Nanoscale Research Letters
Chi-Shuo ChenWei-Chun Chin

Abstract

Human embryonic stem cells [hESCs] are able to differentiate into specific lineages corresponding to regulated spatial and temporal signals. This unique attribute holds great promise for regenerative medicine and cell-based therapy for many human diseases such as spinal cord injury [SCI] and multiple sclerosis [MS]. Carbon nanotubes [CNTs] have been successfully used to promote neuronal differentiation, and silk has been widely applied in tissue engineering. This study aims to build silk-CNT composite scaffolds for improved neuron differentiation efficiency from hESCs.Two neuronal markers (β-III tubulin and nestin) were utilized to determine the hESC neuronal lineage differentiation. In addition, axonal lengths were measured to evaluate the progress of neuronal development. The results demonstrated that cells on silk-CNT scaffolds have a higher β-III tubulin and nestin expression, suggesting augmented neuronal differentiation. In addition, longer axons with higher density were found to associate with silk-CNT scaffolds.Our silk-CNT-based composite scaffolds can promote neuronal differentiation of hESCs. The silk-CNT composite scaffolds developed here can serve as efficient supporting matrices for stem cell-derived neuronal tra...Continue Reading

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Citations

Feb 23, 2013·ACS Applied Materials & Interfaces·Elena HeisterAlan B Dalton
Jan 16, 2013·Biological Procedures Online·Chi-Shuo ChenWei-Chun Chin
Sep 21, 2013·Cellular and Molecular Neurobiology·Wen ZhaoDongsheng Zhang
Jul 17, 2013·Advanced Drug Delivery Reviews·Alessandra FabbroLaura Ballerini
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Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Guorui Jin, Kai Li
Feb 6, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Zheng Jun LvJing Liu
Mar 29, 2014·Journal of Tissue Engineering and Regenerative Medicine·Wei SunAntonella Motta
Nov 26, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Aneta Fraczek-Szczypta
Aug 16, 2016·ACS Applied Materials & Interfaces·Deboki NaskarSubhas C Kundu
Oct 25, 2016·International Journal of Molecular Sciences·Christine Radtke
Apr 11, 2013·Stem Cells Translational Medicine·Marina V Pryzhkova
May 30, 2017·Nanomedicine : Nanotechnology, Biology, and Medicine·Ozlem ErolMustafa O Guler
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Apr 4, 2021·Pharmaceutics·Mahdi YonesiDaniel González-Nieto
Apr 12, 2014·Chemical Reviews·Naoto SaitoMorinobu Endo
Jan 25, 2014·ACS Applied Materials & Interfaces·Eric W BrunnerAlan B Dalton

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Methods Mentioned

BETA
fluorescence microscopy
light microscopy

Software Mentioned

SimplePCI

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