PMID: 3214414Jun 1, 1988Paper

Human stomach alcohol and aldehyde dehydrogenases (ALDH): a genetic model proposed for ALDH III isozymes

Biochemical Genetics
S J YinC W Wu

Abstract

Isozyme phenotypes of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) from human gastroendoscopic as well as surgical gastric biopsies were determined by starch gel electrophoresis and agarose isoelectric focusing. gamma gamma ADH isozymes were expressed predominantly in the mucosal layer of the stomach, whereas beta beta isozymes were in the muscular layer. In the 56 gastroendoscopic mucosal biopsies examined, the homozygous ADH3 1-1 phenotype was found in 75% of the samples, and the heterozygous ADH3 2-1 phenotype in 25%. Accordingly, the gene frequencies of the alleles ADH1/3 and ADH2/3 were calculated to be 0.88 and 0.12, respectively. Using a modified agarose isoelectric focusing procedure, gastric ALDH I, ALDH II, and up to five ALDH III forms could be clearly resolved. The ALDH III isozymes accounted for more than 80% of the total ALDH activities in gastric mucosa and exhibited Km values in the millimolar range for propionaldehyde at pH 9.0. Forty-five percent of the 55 gastroendoscopic biopsies studied lacked ALDH I isozyme. The complex gastric ALDH III isozyme phenotypes seen in these biopsies fall into three patterns. They can be interpreted by a genetic hypothesis, based on a dimeric molecule, in which ...Continue Reading

References

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Citations

Apr 1, 1993·Alcoholism, Clinical and Experimental Research·S J YinS C Lee
Oct 1, 1994·Alcoholism, Clinical and Experimental Research·P KaihovaaraM Salaspuro
Apr 1, 1995·Alcoholism, Clinical and Experimental Research·L E Dyck
Nov 1, 1996·Alcoholism, Clinical and Experimental Research·K E Kitson
Jun 24, 2006·Alcoholism, Clinical and Experimental Research·Shou-Lun LeeShih-Jiun Yin
Apr 8, 1998·Nutrition Reviews·H K Seitz, C M Oneta
Jan 1, 1990·Pharmacology & Therapeutics·D P Agarwal, H W Goedde
Jan 1, 1990·Pharmacology & Therapeutics·H W Goedde, D P Agarwal

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