Human T-cell-porcine endothelial cell interactions induce human Th1 cytokines and porcine activation markers

The Journal of Surgical Research
T S ColemanK M Verbanac

Abstract

Since the future of clinical transplantation will undoubtedly include xenotransplantation, there is a need to examine human anti-pig cellular reactions. The objective of this study is to use human anti-porcine mixed lymphocyte endothelial cell culture (MLEC) to investigate cell interactions, cross-species molecular compatibilities, and the induction of human cytokines and porcine activation markers. Human peripheral blood mononuclear cells or enriched CD4+ T cells depleted of professional antigen-presenting cells were cultured with resting pig aortic endothelial cells in the absence of exogenous cytokines. T-cell proliferative responses were measured and PAEC were monitored for cell surface markers by flow cytometry. Culture supernatants were assayed for human TNF-alpha and IFN-gamma by ELISA. Human T cells proliferated strongly in response to PAEC (median stimulation index = 75), even in serum-free cultures. High levels of the human Th1 cytokines TNF-alpha (20-350 pg/ml) and IFN-gamma (200-3800 pg/ml) were detected only in cultures containing PAEC, with levels peaking on Day 4. CD4+ T-cell-enriched, APC-depleted responders maintained proliferative anti-PAEC responses and cytokine release. By Day 3, MHC Class II and VCAM expres...Continue Reading

References

Jan 15, 1990·Biochemical and Biophysical Research Communications·M YoshizumiY Yazaki
Sep 1, 1995·Nature Medicine·F H BachW W Hancock
Apr 1, 1998·Transplantation·S ShishidoT Mohanakumar

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Citations

Jun 15, 2006·Transplant International : Official Journal of the European Society for Organ Transplantation·Daniel J MooreShaoping Deng
Jun 13, 2002·Xenotransplantation·Soheyla Saadi, Jeffrey L Platt

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