Human T helper cells specific for HIV reverse transcriptase: possible role in intrastructural help for HIV envelope-specific antibodies

European Journal of Immunology
Fabrizio MancaD Saverino

Abstract

Cooperation between B cells specific for an antigen exposed on a viral structure and T helper (Th) cells specific for an internal antigen, as demonstrated with influenza, hepatitis B and rabies viruses, has been termed intrastructural help. Th cells specific for internal proteins of HIV, which are much less mutated than its exposed antigens, may be valuable in vaccine design against this virus. We investigated the human Th repertoire specific for the core HIV antigen reverse transcriptase (p66), and determined whether these cells could be candidate intrastructural T helpers. CD4+ T lines and clones were generated from non-immune individuals by stimulation with p66-pulsed antigen-presenting cells (APC). Specific lines were obtained with p66 from 19 out of 21 (90%) of these individuals, vs. 7 out of 29 (24%) with gp120. Diverse epitopes were recognized by different individuals, and various V beta genes were used by these clones. Clones using the same V beta genes were of diverse origin, according to VDJ region sequence. Of these lines 45% responded to p66 in the context of HIV virions. Moreover, p66-specific clones could respond to APC that had internalized HIV complexed with envelope-specific monoclonal antibodies, suggesting th...Continue Reading

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Citations

Jun 8, 1999·European Journal of Immunology·D FenoglioF Manca
Mar 1, 1996·Immunology Today·M WestbyA G Dalgleish
Mar 1, 1996·Research in Virology·D FenoglioF Manca
Feb 26, 2015·Human Vaccines & Immunotherapeutics·Daniela FenoglioGilberto Filaci
Sep 13, 2012·Journal of Immunological Methods·Giuseppina Li PiraFabrizio Manca
Sep 28, 2005·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Giuseppina Li PiraFabrizio Manca

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