Human T lymphocyte differentiation antigens: effects of blood sample storage on Leu antibody binding

Cytometry
P A HensleighL A Herzenberg

Abstract

Current studies of human T lymphocytes and their subsets often use quantitative immunofluorescence analysis with monoclonal antibodies against cell surface antigens. With storage of whole blood or separated mononuclear cells for more than a few hours we have found marked changes in lymphocyte analysis using a fluorescence activated cell sorter (FACS). Experiments were done to determine if these lymphocyte changes were influenced by storage temperature and if lymphocytes could be made more stable by addition of culture media RPMI 1640 to whole blood. Optimal conditions found for blood storage were with with addition of 50% RPMI 1640 and with samples held at room temperature (22 degrees C). With these storage conditions, delay on FACS analysis up to 24 hours did not result in spurious results. When blood samples are collected in places remote from the laboratory or when batch analysis of serially collected samples is desirable, excessive storage times should be avoided.

References


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Citations

Sep 27, 1993·Journal of Immunological Methods·J K Nicholson, T A Green
Sep 1, 1983·Journal of Reproductive Immunology·H ZolaP C Beverley
Jan 1, 1985·Immunology Letters·S A RobbJ Newsom-Davis
Apr 1, 1990·Journal of Clinical Pathology·G J BrysonR J Collins
Jul 20, 2010·Journal of Immunological Methods·Georgia AfonsoRoberto Mallone
Aug 12, 2008·Cytometry. Part B, Clinical Cytometry·Jean GrantPaul K Wallace
Jul 1, 1989·Clinical Immunology and Immunopathology·A L Landay, K A Muirhead
Jan 1, 1989·Cytometry·B S Edwards, G M Shopp
Jan 1, 1989·Journal of Clinical Laboratory Analysis·C H Miller, N B Levy
Jul 4, 2002·Hematology/oncology Clinics of North America·Roger S RileyGina Romagnoli

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