Human telomeric RNA G-quadruplex response to point mutation in the G-quartets

The Journal of Physical Chemistry. B
Prachi AgarwalaSouvik Maiti

Abstract

Many putative G-quadruplex forming sequences have been predicted to exist in the human genome and transcriptome. As these sequences are subject to point mutations or SNPs (single nucleotide polymorphisms) during the course of evolution, we attempt to understand impact of these mutations in context of RNA G-quadruplex formation using human telomeric RNA (TERRA) as a model sequence. Our studies suggest that G-quadruplex stability is sensitive to substitution of the guanines comprising G-quartets. While central G-quartet plays a crucial role in maintaining the DNA G-quadruplex stability as evident in literature, there is equal importance of three G-quartets in the stability of RNA quadruplex structure. The work here highlights the alterations in the G-quartet are detrimental to the integrity of overall RNA G-quadruplex structure. Furthermore, TmPyP4 molecules are shown to exhibit similar binding behavior toward telomeric RNA G-quadruplex harboring base substitutions employing CD titrations and isothermal titration calorimetry; well indicating that mutation does not influence TmPyP4 recognition ability as it affects the stability of RNA G-quadruplex. Thus, our study implicates that mutation in G-quartets causes destabilization of R...Continue Reading

References

Sep 29, 1998·Methods in Enzymology·L Indyk, H F Fisher
Oct 2, 1998·FEBS Letters·J L MergnyL Lacroix
Oct 21, 1999·Methods : a Companion to Methods in Enzymology·M M PierceB T Nall
Jun 7, 2002·Nature·Gary N ParkinsonStephen Neidle
Jul 2, 2003·Current Opinion in Structural Biology·Stephen Neidle, Gary N Parkinson
Aug 30, 2003·Organic & Biomolecular Chemistry·Antonina Risitano, Keith R Fox
Dec 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·M GELLERTD R DAVIES
May 14, 2004·Nucleic Acids Research·Antonina Risitano, Keith R Fox
Dec 17, 2004·Journal of the American Chemical Society·Pascale HazelStephen Neidle
Jan 12, 2005·Nucleic Acids Research·Jean-Louis MergnyLaurent Lacroix
May 26, 2005·Nucleic Acids Research·Julian L Huppert, Shankar Balasubramanian
Nov 4, 2005·Organic & Biomolecular Chemistry·Nicholas M BrownKeith R Fox
Mar 31, 2006·The Journal of Physical Chemistry. B·Chris M OlsenLuis A Marky
Oct 3, 2006·Nucleic Acids Research·Sarah BurgeStephen Neidle
Feb 6, 2007·Biochemistry·Gary N ParkinsonStephen Neidle
Apr 3, 2007·FEBS Letters·Phillip A RachwalKeith R Fox
Feb 26, 2008·Biochimie·Aurore GuédinJean-Louis Mergny
Apr 9, 2008·Biochimie·Stephen Neidle, Gary N Parkinson
Jun 17, 2008·The Journal of Physical Chemistry. B·Amit Arora, Souvik Maiti
Jul 5, 2008·Nucleic Acids Research·Niti KumarSouvik Maiti
Jul 23, 2008·Journal of the American Chemical Society·Yan XuMakoto Komiyama
Oct 4, 2008·Nucleic Acids Research·Julian Leon HuppertShankar Balasubramanian
Feb 3, 2009·Journal of the American Chemical Society·Herry Martadinata, Anh Tuân Phan
Feb 7, 2009·The Journal of Physical Chemistry. B·Chris M Olsen, Luis A Marky
Jul 4, 2009·The Journal of Physical Chemistry. B·Amit Arora, Souvik Maiti
Jul 8, 2009·Nucleic Acids Research·Aurore GuédinJean-Louis Mergny
Oct 15, 2009·The Journal of Physical Chemistry. B·Luigi MartinoConcetta Giancola
Dec 3, 2009·The FEBS Journal·Anh Tuân Phan
Apr 24, 2010·Nucleic Acids Research·Gavin W CollieGary N Parkinson
Jan 20, 2012·RNA Biology·Gopal Gunanathan JayarajSouvik Maiti
Apr 30, 2013·Biochemistry·Chun Kit KwokPhilip C Bevilacqua
May 21, 2013·The Journal of Physical Chemistry. B·Satyaprakash PandeySouvik Maiti

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Citations

Apr 17, 2015·Organic & Biomolecular Chemistry·Prachi AgarwalaSouvik Maiti
Oct 30, 2016·Nucleic Acids Research·Kateřina ŠvehlováEdward A Curtis
Jul 22, 2019·FEBS Letters·Helen L LightfootJonathan Hall
May 29, 2018·Journal of Biomolecular Structure & Dynamics·Zhiguo WangJun-Ping Liu
May 19, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jia-Yuan GongZheng Tan

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