Human testis expresses a specific poly(A)-binding protein

Nucleic Acids Research
C FéralA Pawlak

Abstract

In testis mRNA stability and translation initiation are extensively under the control of poly(A)-binding proteins (PABP). Here we have cloned a new human testis-specific PABP (PABP3) of 631 amino acids (70.1 kDa) with 92.5% identical residues to the ubiquitous PABP1. A northern blot of multiple human tissues hybridised with PABP3- and PABP1-specific oligonucleotide probes revealed two PABP3 mRNAs (2.1 and 2.5 kb) detected only in testis, whereas PABP1 mRNA (3.2 kb) was present in all tested tissues. In human adult testis, PABP3 mRNA expression was restricted to round spermatids, whereas PABP1 was expressed in these cells as well as in pachytene spermatocytes. PABP3-specific antibodies identified a protein of 70 kDa in human testis extracts. This protein binds poly(A) with a slightly lower affinity as compared to PABP1. The human PABP3 gene is intronless with a transcription start site 61 nt upstream from the initiation codon. A sequence of 256 bp upstream from the transcription start site drives the promoter activity of PABP3 and its tissue-specific expression. The expression of PABP3 might be a way to bypass PABP1 translational repression and to produce the amount of PABP needed for active mRNA translation in spermatids.

References

Jul 11, 1992·Nucleic Acids Research·M Y WangK C Kleene
Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D J Wolgemuth, F Watrin
Jul 25, 1991·Nucleic Acids Research·R H DonJ S Mattick
Jan 1, 1989·Molecular Reproduction and Development·P C YelickN B Hecht
Jan 1, 1986·Annual Review of Cell Biology·G Dreyfuss
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G Blobel
Jun 25, 1995·Nucleic Acids Research·O P de Melo NetoC Martins de Sa
Mar 1, 1995·Molecular Reproduction and Development·W GuN B Hecht
Dec 1, 1994·Molecular Reproduction and Development·K C KleeneD Shih
Jan 1, 1993·Annual Review of Biochemistry·G DreyfussC G Burd
Dec 1, 1995·Developmental Biology·M SchäferU Schäfer
Apr 1, 1996·European Journal of Biochemistry·J Bag, J Wu
May 28, 1998·The Journal of Biological Chemistry·E AfoninaG N Pavlakis
Aug 26, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A PawlakG Guellaën
Nov 11, 1998·Molecular Human Reproduction·K StegerM Bergmann
Mar 20, 2003·Academic Radiology·J Shannon SwanDennis M Heisey

❮ Previous
Next ❯

Citations

Aug 7, 2002·Journal of Molecular Biology·Neva WestPrescott Deininger
Aug 7, 2002·Journal of Molecular Biology·Ravi MuddashettyJürgen Brosius
Jun 13, 2006·Bioinformatics·Isabelle Dupanloup, Henrik Kaessmann
Jul 26, 2008·Molecular Biology and Evolution·Steve DorusTimothy L Karr
Aug 22, 2008·Molecular Human Reproduction·Ozlem Guzeloglu-KayisliEmre Seli
Feb 17, 2005·Molecular and Cellular Biology·Gavin S WilkieNicola K Gray
Apr 4, 2006·Molecular and Cellular Biology·Nao HosodaLynne E Maquat
Mar 9, 2012·Comparative and Functional Genomics·Sarah K Darmon, Carol S Lutz
Oct 6, 2005·PLoS Biology·Ana Claudia MarquesHenrik Kaessmann
Feb 20, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Matthew BrookNicola K Gray
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Nicolas VinckenboschHenrik Kaessmann
Jan 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Emre SeliJoan A Steitz
Feb 12, 2005·Reproductive Biomedicine Online·Ingrid Ehrmann, David J Elliott
Jan 29, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kentaro OkochiTadashi Yamamoto
May 26, 2004·Biochimica Et Biophysica Acta·Uwe Kühn, Elmar Wahle
Jul 15, 2015·Applied Biochemistry and Biotechnology·Shanshan LiuHanjiang Fu
Mar 15, 2016·The Journal of Reproduction and Development·Shin-Ichi KashiwabaraTadashi Baba
Jul 21, 2012·Reproductive Sciences·Saffet OzturkEmre Seli
May 22, 2018·The Journal of Reproduction and Development·Saffet Ozturk, Fatma Uysal
Jan 20, 2017·Reproduction, Fertility, and Development·Saffet Ozturk, Fatma Uysal
Apr 10, 2002·The Journal of Biological Chemistry·Guennadi KozlovKalle Gehring
Jun 4, 2004·The Journal of Biological Chemistry·Cercina OnestoGilles Pagès
Feb 9, 2016·Jundishapur Journal of Microbiology·Tzu Shan NgLeslie Thian Lung Than
Mar 10, 2015·International Journal of Endocrinology and Metabolism·Zahra DaneshianAzita Zadeh Vakili
Jun 7, 2015·Cancer Immunology Research·Antonia L PritchardChristopher W Schmidt
Apr 9, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Long-Wen Zhao, Heng-Yu Fan
Aug 24, 2021·Future Oncology·Tailai AnChanghua Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.