Human thymic dendritic cell-thymocyte association: ultrastructural cell phenotype analysis.

European Journal of Immunology
D LandryS Montplaisir

Abstract

In rodent thymus, associations between dendritic cells (DC) and thymocytes have been suggested to be implicated in differentiation and/or maturation processes. In this study, we report intimate associations formed between human thymic DC and thymocytes in culture and we analyze their ultrastructural cell phenotype. Observations by phase contrast microscopy showed that DC present long and thin dendrites and bind many thymocytes. Transmission (TEM) and scanning electron microscopy (SEM) revealed that both cellular populations were in close connection and tight membrane contact could be observed. The phenotype of DC and attached thymocytes was characterized with a series of monoclonal antibodies by protein A-gold TEM and SEM immunolabelings. Quantitative evaluation of immunolabeling (number of gold granules/microns of cellular membrane) suggests the presence of two subpopulations of CD1+ thymic DC (strong and weak), whereas this discrepancy is not observed in DR+ and CD4+ DC populations. On the other hand, the majority of thymocytes bound to DC strongly express the CD1, CD4, CD8 and CD2 antigens and weakly the CD3 antigen, indicating that they represent double-positive immature thymocytes. Uniform distribution of DC and thymocytes...Continue Reading

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Citations

Mar 27, 1995·Journal of Immunological Methods·S BeaulieuS Montplaisir
Sep 1, 1993·Immunology Today·R L BoydP Hugo
Sep 1, 1993·Immunology Today·M A Ritter, R L Boyd
Mar 22, 2000·Acta histochemica·A Seftalioğlu, L Karakoç
Feb 1, 1991·Immunology Today·R L Boyd, P Hugo
Aug 1, 1997·Microscopy Research and Technique·M LafontaineS Montplaisir
Mar 1, 1992·European Journal of Immunology·C Ardavin, K Shortman
Jan 1, 1990·International Reviews of Immunology·K InabaM Inaba

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