Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens

Immunology and Cell Biology
Gabriel SkogbergOlov Ekwall

Abstract

Exosomes are nano-sized vesicles released by cells into the extracellular space and have been shown to be present in thymic tissue both in mice and in humans. The source of thymic exosomes is however still an enigma and hence it is not known whether thymic epithelial cells (TECs) are able to produce exosomes. In this work, we have cultured human TECs and isolated exosomes. These exosomes carry tissue-restricted antigens (TRAs), for example, myelin basic protein and desmoglein 3. The presence of TRAs indicates a possible role for thymic epithelium-derived exosomes in the selection process of thymocytes. The key contribution of these exosomes could be to disseminate self-antigens from the thymic epithelia, thus making them more accessible to the pool of maturing thymocytes. This would increase the coverage of TRAs within the thymus, and facilitate the process of positive and negative selection.

References

Oct 1, 1990·Arthritis and Rheumatism·K TeratoM Goto
Mar 1, 1996·The Journal of Experimental Medicine·G RaposoH J Geuze
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Feb 1, 1997·Journal of Cellular Biochemistry·N JaiswalS P Bruder
Aug 1, 1997·Microscopy Research and Technique·C Röpke
Aug 7, 2001·Gastroenterology·G van NielM Heyman
Mar 22, 2002·Nature Reviews. Immunology·G Anderson, E J Jenkinson
Jun 27, 2002·Biochemical and Biophysical Research Communications·Yoon LimDoo-Il Jeoung
Jul 4, 2002·International Immunology·Hélène Vincent-SchneiderChristian Bonnerot
May 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Inkyu HwangJonathan Sprent
Oct 1, 2003·Nature Immunology·Robert K ChinYang-Xin Fu
Jun 29, 2005·The Journal of Experimental Medicine·Jens DerbinskiBruno Kyewski
Jun 9, 2006·European Journal of Immunology·Charlotte AdmyreSusanne Gabrielsson
Aug 23, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Shingo Ichimiya, Takashi Kojima
Aug 29, 2006·Diabetes·Michael O PalumboConstantin Polychronakos
Jan 20, 2007·Journal of Clinical Immunology·Agnieszka MagrysGrazyna Adamus
Jun 30, 2007·Molecular Immunology·Vivian KontPärt Peterson
Jan 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jens DerbinskiBruno Kyewski
Jan 23, 2008·Archives of Dermatology·Yohei IwataShinichi Sato
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Oct 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gui-Jun WangHuang-Ge Zhang
Jul 1, 2009·The Journal of Experimental Medicine·Christian Koble, Bruno Kyewski
Dec 17, 2009·Nature Reviews. Immunology·Marcus GroettrupMichael Basler
Feb 17, 2010·Immune Network·Kwon Ik Oh, Jae Nam Seo
May 29, 2010·The Journal of Dermatology·Teruki DainichiTakashi Hashimoto
Jul 6, 2010·Journal of Proteomics·Suresh MathivananRichard J Simpson
Oct 19, 2011·The European Respiratory Journal·Hanadi DibLuc Mouthon
Dec 23, 2011·The Journal of Investigative Dermatology·Masayuki Amagai, John R Stanley
Dec 28, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sheena PintoBruno Kyewski
Jul 12, 2013·PloS One·Gabriel SkogbergOlov Ekwall
Jan 18, 2014·Autoimmunity Reviews·Alberto FalorniRoberto Gerli
May 17, 2014·Nature Reviews. Immunology·Ludger KleinKristin A Hogquist
Jul 20, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gabriel SkogbergOlov Ekwall

❮ Previous
Next ❯

Citations

Feb 19, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Elena V Batrakova, Myung Soo Kim
Jul 4, 2015·Immune Network·Jaehak Oh, Jeoung-Sook Shin
Aug 5, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Elena V Batrakova, Myung Soo Kim
Apr 1, 2016·Molecular BioSystems·Agata AbramowiczMonika Pietrowska
Aug 11, 2015·Frontiers in Immunology·Noëlla LopesMagali Irla
Aug 11, 2015·Frontiers in Immunology·Gabriel SkogbergOlov Ekwall
Mar 31, 2016·PloS One·Hong-Xia WangXiao-Ping Zhong
Dec 7, 2017·Journal of Extracellular Vesicles·Chuan WenAmbrose Y Jong
Jun 3, 2015·Immunology and Cell Biology·Julie Sheridan, Daniel Gray
May 22, 2019·Frontiers in Immunology·Krisztina BanfaiKrisztian Kvell
Jul 30, 2019·Frontiers in Cellular and Infection Microbiology·Monica Losada-BarragánPatricia Cuervo
Feb 28, 2018·Frontiers in Immunology·Roberto Perniola
Jan 5, 2021·Frontiers in Pharmacology·Zhilong ZhaoCongxiao Zhang
Mar 18, 2021·Disease Models & Mechanisms·Marine BesnardCarole Guillonneau
Mar 20, 2021·Frontiers in Cell and Developmental Biology·Hang SongJinghui Wang
Oct 26, 2021·The Journal of Allergy and Clinical Immunology·Rafael Gras-PeñaMegan Sykes

❮ Previous
Next ❯

Methods Mentioned

BETA
dynamic light scattering
flow cytometry
PCR
FCS
protein assay
flow-cytometry
FACS
PCRs
acetylation

Software Mentioned

FlowJo
MASCOT
Nanoparticle Tracking Analysis

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.