Human thymocytes do not respond to interleukin-2 after removal of mature "bright" CD5 positive cells.

Cellular Immunology
Y UenoChristel H Uittenbogaart

Abstract

Interleukin-2 receptors (IL-2R) are expressed on minor populations of immature and mature human thymocytes. These studies were designed to determine if immature T cells could respond to the mitogen phytohemagglutinin (PHA-P) plus IL-2 in vitro by increasing the expression of IL-2R and by proliferation. Using monoclonal antibodies to CD5 and magnetic immunobeads we were able to remove all mature, "bright" CD5+ cells from nylon wool-purified thymocytes and to obtain less mature cells which consisted almost completely of cells with the CD4+CD8+ phenotype. These immature cells were mostly "dim" CD5+ and less than 5% CD5- and a small percentage expressed the IL-2R. After culture in serum-free medium with PHA-P, these cells showed only a slight increase in the percentage of IL-2R+ cells and the addition of IL-2 did not increase the percentage of IL-2R+ cells and no proliferation was observed. Unseparated, nylon wool-purified thymocytes contained 14% bright CD5+ cells. These bright CD5+ cells had a mature phenotype of CD4+CD8- (52%) and CD4-CD8+ (27%) cells. A small percentage of these cells were IL-2R+. These bright CD5+IL-2R+ cells were predominantly mature CD4+CD8- cells as measured by three-color flow cytometry. After culture with...Continue Reading

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Citations

Jan 1, 1991·International Journal of Immunopharmacology·G Sandberg, U Ernström
Apr 1, 1994·Current Opinion in Immunology·H Spits
Feb 1, 1994·Research in Immunology·H SpitsA Galy
Aug 10, 2000·AIDS·C H UittenbogaartJ A Zack
Jun 20, 2002·Journal of Virology·Livia Pedroza-MartinsChristel H Uittenbogaart
Oct 6, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Vanessa A EvansSharon R Lewin
Mar 1, 1995·Leukemia & Lymphoma·C SchmittP Debré
Sep 1, 1995·European Journal of Immunology·S KtorzaC Schmitt
Nov 13, 1998·Journal of Virology·C H UittenbogaartE F Hays

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