PMID: 9177772May 15, 1997Paper

Human TNF receptor-associated factor 5 (TRAF5): cDNA cloning, expression and assignment of the TRAF5 gene to chromosome 1q32

Genomics
H NakanoK Okumura

Abstract

Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) are signal transducers for members of the TNF receptor superfamily. We previously identified murine TRAF5 (mTRAF5) and showed that it specifically interacts with the lymphotoxin-beta receptor (LT-beta R) and activates the transcription factor NF-kappa B. Here we have cloned the human TRAF5 homologue (hTRAF5) by cross hybridization with mTRAF5 cDNA. hTRAF5 cDNA is composed of 2894 nucleotides with a 557-amino-acid open reading frame that exhibits 77.5 and 80% identity to mTRAF5 at the nucleotide and amino acid levels, respectively. Northern blot analysis revealed that hTRAF5 mRNA is expressed in all visceral organs. Western blotting revealed that hTRAF5 protein was abundantly expressed in the human follicular dentritic cell line, FDC-1, and to a much lesser degree in several tumor cell lines. Interspecific backcross mapping revealed that Traf5 is located in the distal region of mouse chromosome 1, which shares a region of homology with human chromosome 1q. Fluorescence in situ hybridization confirmed regional localization to human chromosome 1q32.

References

Apr 1, 1991·Trends in Genetics : TIG·N G Copeland, N A Jenkins
Mar 10, 1995·Science·G ChengD Baltimore
May 19, 1995·Cell·J L Cleveland, J N Ihle
Jan 1, 1995·Current Topics in Microbiology and Immunology·C F WareJ L Browning
Feb 24, 1995·Cell·D Thanos, T Maniatis
Apr 29, 1994·Science·B Beutler, C van Huffel
Apr 29, 1994·Science·P D CroweC A Smith
Jan 1, 1994·Annual Review of Medicine·K J Tracey, A Cerami
Jun 21, 1996·The Journal of Biological Chemistry·H NakanoK Okumura
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·T K IshidaJ Inoue
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·S Y LeeY Choi
Jan 1, 1996·Advances in Immunology·C Van Kooten, J Banchereau
Oct 3, 1996·Nature·Z CaoD V Goeddel
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·R EttingerH O McDevitt
Nov 1, 1996·The Journal of Experimental Medicine·P D RennertP S Hochman
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·T L VanArsdaleC F Ware

❮ Previous
Next ❯

Citations

Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·H NakanoK Okumura
Jun 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jan StaelensClaude Libert
Nov 21, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L RobbA G Elefanty

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.