Human tonsillar tissue block cultures differ from autologous tonsillar cell suspension cultures in lymphocyte subset activation and cytokine gene expression

Journal of Immunological Methods
Bettina GigerDavid Nadal

Abstract

Lymphoid tissues cultured either as tissue blocks or as cell suspensions are used to study the behaviour of immune cells within their habitat. The preservation of tissue structures in tissue blocks, which is considered to be a major advantage, has been poorly defined. We characterised the morphological evolution of tissue cultures from human palatine tonsils and compared their lymphocyte subsets and the constitutive cytokine gene expression to those in autologous tonsillar single-cell suspension cultures over time, and after adding cyclosporin A (CsA) to mimic the situation in individuals treated with immunosuppressive drugs. Density and morphology of follicles were conserved up to 4 days, during which tissue cultures exhibited similar cell viability as suspension cultures, but a significantly less frequent increase of CD95 expression in T cells, smaller variation of the proportion of CD4(+) cells and better CD21(+)/CD23(-) B-cell survival. Treatment with cyclosporin A at higher concentrations resulted in superior histologic preservation of lymphoid tissue structures and seemed to further prevent the expression of CD95 by CD3(+) cells and the activation in tissue culture of CD21(+) cells. Constitutive gene expression levels of ...Continue Reading

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Citations

Jan 20, 2011·The Journal of Clinical Investigation·Lynn M HassmanDean H Kedes
Sep 24, 2010·PloS One·Beda BrichacekLeonid Margolis
Dec 24, 2013·Pediatric Surgery International·Sophie Böttcher-HaberzethMartin Meuli
Sep 8, 2019·Journal of Otolaryngology - Head & Neck Surgery = Le Journal D'oto-rhino-laryngologie Et De Chirurgie Cervico-faciale·Lumei LiuTendy Chiang
Feb 23, 2021·ACS Pharmacology & Translational Science·Maura C BelangerRebecca R Pompano
Apr 20, 2021·Annual Review of Biomedical Engineering·Jennifer H HammelRebecca R Pompano
Dec 14, 2021·Molecular Metabolism·Mohsen Khosravi-MaharlooeiRemi J Creusot

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