Human trabecular bone cells are able to express both osteoblastic and adipocytic phenotype: implications for osteopenic disorders

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
M E NuttallM Gowen

Abstract

The decrease in bone volume associated with osteoporosis and age-related osteopenia is accompanied by increased marrow adipose tissue formation. Reversal of this process may provide a novel therapeutic approach for osteopenic disorders. We have shown that cells cultured from human trabecular bone are not only osteogenic, but are able also to undergo adipocyte differentiation under defined culture conditions. Osteoblast differentiation was induced by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and adipocyte differentiation by dexamethasone (dex) plus 3-isobutyl-1-methylxanthine (IBMX) treatment. Adipogenesis was characterized by lineage-specific enzyme and gene activities, alpha-glycerophosphate-3-dehydrogenase activity, fatty acid binding protein, aP2 and lipoprotein lipase expression. Osteoblastogenesis was assessed by osteoblast characteristic 1,25(OH)2D3 induction of alkaline phosphatase activity and osteoblast-specific 1,25(OH)2D3-induced osteocalcin synthesis and release. We provide evidence for a common pluripotent mesenchymal stem cell that is able either to undergo adipogenesis or osteoblastogenesis, using clonal cell lines derived from human trabecular bone cell cultures. Adipogenesis can be induced also by long chain fatty...Continue Reading

References

Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·T R Russell, R Ho
Nov 26, 1979·Calcified Tissue International·B G MillsP A Holst
Jan 1, 1979·American Journal of Hematology·A BathijaS Trubowitz
Sep 1, 1992·Journal of Cellular Biochemistry·J M GimbleC S Wang
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P A GrimaldiN A Abumrad
Dec 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P GarneroP D Delmas
Jan 1, 1992·Annual Review of Nutrition·G AilhaudR Négrel
Oct 1, 1992·Diabetes·K R Feingold, C Grunfeld
Sep 1, 1991·Calcified Tissue International·A Yamaguchi, A J Kahn
Oct 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G S SteinT A Owen
Aug 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M WeinrebG A Rodan
Sep 1, 1985·Calcified Tissue International·P G Robey, J D Termine
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A Ignotz, J Massagué
Jan 1, 1988·Journal of Cell Science. Supplement·M Owen
Jan 1, 1988·Journal of Cellular Physiology·A HiragunH Mitsui
Jan 1, 1985·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·H A NeumannG W Löhr
Jan 1, 1974·Calcified Tissue Research·P MinaireJ Bourret
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jul 1, 1982·Journal of Cellular Physiology·H A KodamaS Kasai
Jul 1, 1995·Journal of Cellular Biochemistry·J M GimbleV Rosen
Jan 1, 1995·Molecular and Cellular Biology·P TontonozB M Spiegelman
May 15, 1994·Genes & Development·P TontonozB M Spiegelman
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·S A KliewerR M Evans
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·F T Lin, M D Lane

❮ Previous
Next ❯

Citations

May 1, 2007·Regenerative Medicine·Pierre J Marie, Olivia Fromigué
Nov 6, 2012·Clinical and Experimental Medicine·Yi LiuJinxiang Han
Aug 13, 2013·Cellular and Molecular Life Sciences : CMLS·Agnes D Berendsen, Bjorn R Olsen
Mar 14, 2002·Biochemical and Biophysical Research Communications·Felicity R A J Rose, Richard O C Oreffo
May 25, 2001·Analytical Biochemistry·V Sottile, K Seuwen
Mar 13, 2014·Current Osteoporosis Reports·Hiromu Ito
Jul 16, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Kang-Yung PengRong-Tsun Wu
Apr 5, 2014·Archivum Immunologiae Et Therapiae Experimentalis·Yanqiu Liu, Bjorn R Olsen
Sep 23, 2003·Endocrinology·S O RzoncaB Lecka-Czernik
Mar 17, 2004·Molecular and Cellular Biology·Marie KveiborgRoland Baron
Apr 7, 2004·The Journal of Clinical Investigation·Toru AkuneHiroshi Kawaguchi
Jan 30, 2007·PPAR Research·Ann V Schwartz
Apr 3, 2008·The Journal of Clinical Endocrinology and Metabolism·Natascia Di IorgiVicente Gilsanz
Dec 11, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yan-Hsiung WangGwo-Jaw Wang
Apr 28, 2009·Critical Reviews in Eukaryotic Gene Expression·Clifford J RosenAna Maria Pino
Aug 13, 2009·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Weon Wook ParkJung Sub Lee
Dec 17, 2009·The Journal of Biological Chemistry·J Christopher FrittonShoshana Yakar
Apr 16, 2010·The Journal of Clinical Endocrinology and Metabolism·Natascia Di IorgiVicente Gilsanz
Sep 30, 2010·The Journal of Clinical Investigation·Laurie K McCauley
Oct 21, 2010·Stem Cell Research & Therapy·Stephane Boeuf, Wiltrud Richter
Feb 25, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Katherine J MotylLaura R McCabe
May 13, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J E DennisA I Caplan
Dec 10, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Grahame Elder
May 15, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Andrea Plant, Jonathan H Tobias
Jan 5, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·F ParhamiL L Demer
Nov 25, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·F S Kaplan, E M Shore
Nov 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C G Bellows, J N Heersche
Jan 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ken-Ichi TezukaNobumichi Hozumi
Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J KawaguchiA Kudo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Related Papers

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Souhila AhdjoudjP J Marie
FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Lin Song, Rocky S Tuan
Aging Clinical and Experimental Research
Chiara RattiPaolo Cherubino
© 2022 Meta ULC. All rights reserved