Human tumor cell proliferation evaluated using manganese-enhanced MRI.

PloS One
Rod D BraunBruce A Berkowitz

Abstract

Tumor cell proliferation can depend on calcium entry across the cell membrane. As a first step toward the development of a non-invasive test of the extent of tumor cell proliferation in vivo, we tested the hypothesis that tumor cell uptake of a calcium surrogate, Mn(2+) [measured with manganese-enhanced MRI (MEMRI)], is linked to proliferation rate in vitro. Proliferation rates were determined in vitro in three different human tumor cell lines: C918 and OCM-1 human uveal melanomas and PC-3 prostate carcinoma. Cells growing at different average proliferation rates were exposed to 1 mM MnCl(2) for one hour and then thoroughly washed. MEMRI R(1) values (longitudinal relaxation rates), which have a positive linear relationship with Mn(2+) concentration, were then determined from cell pellets. Cell cycle distributions were determined using propidium iodide staining and flow cytometry. All three lines showed Mn(2+)-induced increases in R(1) compared to cells not exposed to Mn(2+). C918 and PC-3 cells each showed a significant, positive correlation between MEMRI R(1) values and proliferation rate (p≤0.005), while OCM-1 cells showed no significant correlation. Preliminary, general modeling of these positive relationships suggested that...Continue Reading

References

Jun 1, 1983·The Journal of General Physiology·M Anderson
Oct 27, 1997·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Y J Lin, A P Koretsky
Oct 28, 1998·Nature·M J BerridgeP Lipp
May 29, 2002·Nuclear Medicine and Biology·Haruna TamanoAtsushi Takeda
Jan 1, 2003·The Journal of General Physiology·Mahealani K Monteilh-ZollerAndrea Fleig
Feb 5, 2003·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·R M ConwayL M Holbach
Apr 4, 2003·The Journal of Biological Chemistry·Christian GrimmChristian Harteneck
Apr 20, 2004·Biology of the Cell·Larissa Lipskaia, Anne-Marie Lompré
Jun 8, 2004·Current Medicinal Chemistry·Luca MunaronDavide Lovisolo
Jul 17, 2004·Journal of Cellular and Molecular Medicine·L MunaronD Lovisolo
Sep 30, 2004·International Journal of Food Microbiology·S LópezJ France
Dec 24, 2004·NMR in Biomedicine·Alan P Koretsky, Afonso C Silva
Mar 12, 2005·NMR in Biomedicine·Youssef Zaim WadghiriDaniel H Turnbull
Jun 1, 2005·Nature Neuroscience·Xin YuDaniel H Turnbull
Sep 30, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ander UrruticoecheaMitch Dowsett
May 26, 2006·Investigative Ophthalmology & Visual Science·Bruce A BerkowitzHongmei Luan
Jun 9, 2006·Diagnostic Pathology·A BuhmeidaY Collan
Jun 13, 2006·Cell Calcium·Amith Panner, Robert D Wurster
Nov 7, 2006·Pharmacology & Therapeutics·Keith M EriksonMichael Aschner
Nov 18, 2006·Cancer Metastasis Reviews·Jamie L FiskeRobert A Sikes
Feb 28, 2007·Investigative Ophthalmology & Visual Science·Rod D BraunBruce A Berkowitz
Mar 27, 2007·NeuroImage·Debasish BanerjeeJoel R Garbow
Oct 11, 2007·AJNR. American Journal of Neuroradiology·D J CrossS Minoshima
Jul 1, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·James R Bading, Anthony F Shields
Sep 3, 2008·World Journal of Gastroenterology : WJG·James T TaylorMing Li
Dec 9, 2008·Investigative Ophthalmology & Visual Science·Bruce A BerkowitzMarius Gradianu
Mar 12, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A BuhmeidaS Pyrhönen
Feb 25, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sarah MinnerThorsten Schlomm
Jul 6, 2010·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Shinichi Hasegawa, Takaki Yoshikawa
Jul 10, 2010·International Journal of Cancer. Journal International Du Cancer·Sumitaka HasegawaTsuneo Saga
Sep 2, 2010·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Mukund Seshadri, Allison Hoy
Jun 12, 2012·The Journal of Urology·Anthony Atala

❮ Previous
Next ❯

Citations

Mar 5, 2014·Journal of Magnetic Resonance Imaging : JMRI·Tameshwar GaneshHai-Ling Margaret Cheng
Jul 25, 2015·Nanoscale·Vasudevanpillai BijuShin-ichi Wakida
Dec 21, 2016·Scientific Reports·Charles R CastetsEmeline J Ribot
Mar 14, 2014·Molecular Imaging·Joris Tchouala NofieleHai-Ling Margaret Cheng
Jan 1, 2017·European Radiology Experimental·Liang WenDan Han
Sep 1, 2017·NMR in Biomedicine·Andrea BianchiYannick Crémillieux
May 23, 2013·Cancer Research·Shigeyoshi SaitoIchio Aoki

❮ Previous
Next ❯

Methods Mentioned

BETA
xenografts
flow cytometry

Software Mentioned

lm
GraphPad
MEMRI
ImageJ
minpack
GraphPad Prism
ModFit LT

Related Concepts

Related Feeds

Related Papers

Magnetic Resonance in Medical Sciences : MRMS : an Official Journal of Japan Society of Magnetic Resonance in Medicine
Sumitaka HasegawaTsuneo Saga
Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Mukund Seshadri, Allison Hoy
© 2022 Meta ULC. All rights reserved