Abstract
Although human pluripotent stem cells (hPSCs) can proliferate robustly on the feeder-free culture system, genetic instability of hPSCs has been reported in such environment. Alternatively, feeder cells enable hPSCs to maintain their pluripotency. The feeder cells are usually grown in a culture medium containing fetal bovine serum (FBS) prior to coculture with hPSCs. The use of FBS might limit the clinical application of hPSCs. Recently, human cord blood-derived serum (hUCS) showed a positive effect on culture of mesenchymal stem cells. It is interesting to test whether hUCS can be used for culture of feeder cells of hPSCs. This study was aimed to replace FBS with hUCS for culturing the human foreskin fibroblasts (HFFs) prior to feeder cell preparation. The results showed that HFFs cultured in hUCS-containing medium (HFF-hUCS) displayed fibroblastic features, high proliferation rates, short population doubling times, and normal karyotypes after prolonged culture. Inactivated HFF-hUCS expressed important genes, including Activin A, FGF2, and TGFβ1, which have been implicated in the maintenance of hPSC pluripotency. Moreover, hPSC lines maintained pluripotency, differentiation capacities, and karyotypic stability after being cocul...Continue Reading
References
Nov 6, 1998·Science·J A ThomsonJ M Jones
Jul 2, 2003·Human Reproduction·Outi HovattaLars Ahrlund-Richter
Mar 26, 2005·Stem Cells·José InzunzaOuti Hovatta
Jan 3, 2006·Nature Biotechnology·Tenneille E LudwigJames A Thomson
Jun 3, 2006·Stem Cells·Catharina EllerströmHenrik Semb
Nov 1, 2006·Cell Biology International·Ying-Tang ShiJian-Xin Chu
Jan 9, 2007·Cell Biology International·P ShettyV Tanavde
Jan 27, 2007·Stem Cells·Asli KocaoemerKaren Bieback
Nov 24, 2007·Cell·Kazutoshi TakahashiShinya Yamanaka
Mar 5, 2008·Molecular Reproduction and Development·Shobhit SaxenaSatish Totey
Apr 17, 2008·The International Journal of Developmental Biology·Livia EiselleovaPetr Dvorak
Aug 30, 2008·Stem Cells and Development·Jooyoung JungIl-Hoan Oh
Oct 7, 2008·Molecular Cancer·Puri CatalinaPablo Menendez
Jun 27, 2009·Human Reproduction·Christian UngerAnis Feki
Nov 4, 2009·Stem Cells and Development·Guoliang MengDerrick E Rancourt
Jun 25, 2010·Tissue Engineering. Part a·Karen BiebackPeter Bugert
Feb 22, 2011·Journal of Cellular Biochemistry·Murali Krishna MamidiRamesh Bhonde
Mar 11, 2011·Nature Reviews. Cancer·Uri Ben-David, Nissim Benvenisty
Mar 23, 2011·Journal of Cellular and Molecular Medicine·Hanae UeyamaKoji Kawakami
Nov 15, 2011·International Review of Cell and Molecular Biology·Elena S PhilonenkoSergey L Kiselev
Dec 14, 2011·Stem Cells and Development·Guoliang MengDerrick E Rancourt
Jan 28, 2012·Lancet·Steven D SchwartzRobert Lanza
Apr 5, 2012·Stem Cell Research & Therapy·Nilendran PrathalingamMary Herbert
May 17, 2012·Cytotherapy·Parvin SalehinejadMasoomeh Hajghani
Jun 30, 2012·PloS One·Shelly E TannenbaumBenjamin E Reubinoff
Mar 22, 2013·BioResearch Open Access·Kamthorn PruksananondaPramuan Virutamasen
May 10, 2013·The Journal of Biological Chemistry·Despina SoteriouSusan J Kimber
Jun 15, 2013·Head & Neck·Nicole Y K LiSusan L Thibeault
Oct 8, 2013·PloS One·Sanna VuoristoTimo Otonkoski
Oct 15, 2013·Cytotherapy·Suphakde JulavijitphongTatsanee Phermthai
Jan 1, 2013·Acta Medica (Hradec Králové)·Jakub SuchánekTomás Soukup
Dec 3, 2014·Lancet·Steven D SchwartzRobert Lanza
Dec 19, 2014·Thrombosis and Haemostasis·Praewphan IngrungruanglertVorasuk Shotelersuk
Citations
Apr 6, 2016·Archives of Oral Biology·Stefanie Bressan WerleLuciano Casagrande
Sep 4, 2016·Human Reproduction Update·Teena Kjb GamageJoanna L James
Feb 16, 2019·Regenerative Medicine·Eduardo AnituaMaría Troya
Jul 1, 2020·Cytotechnology·Maryam Samareh Salavati PourAlireza Farsinejad
Feb 6, 2021·Human Cell·Ruttachuk RungsiwiwutKamthorn Pruksananonda
Aug 20, 2021·Stem Cell Reviews and Reports·Deepa BhartiyaAnkita Kaushik