Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines

Stem Cells International
Ruttachuk RungsiwiwutKamthorn Pruksananonda

Abstract

Although human pluripotent stem cells (hPSCs) can proliferate robustly on the feeder-free culture system, genetic instability of hPSCs has been reported in such environment. Alternatively, feeder cells enable hPSCs to maintain their pluripotency. The feeder cells are usually grown in a culture medium containing fetal bovine serum (FBS) prior to coculture with hPSCs. The use of FBS might limit the clinical application of hPSCs. Recently, human cord blood-derived serum (hUCS) showed a positive effect on culture of mesenchymal stem cells. It is interesting to test whether hUCS can be used for culture of feeder cells of hPSCs. This study was aimed to replace FBS with hUCS for culturing the human foreskin fibroblasts (HFFs) prior to feeder cell preparation. The results showed that HFFs cultured in hUCS-containing medium (HFF-hUCS) displayed fibroblastic features, high proliferation rates, short population doubling times, and normal karyotypes after prolonged culture. Inactivated HFF-hUCS expressed important genes, including Activin A, FGF2, and TGFβ1, which have been implicated in the maintenance of hPSC pluripotency. Moreover, hPSC lines maintained pluripotency, differentiation capacities, and karyotypic stability after being cocul...Continue Reading

References

Nov 6, 1998·Science·J A ThomsonJ M Jones
Jan 3, 2006·Nature Biotechnology·Tenneille E LudwigJames A Thomson
Jun 3, 2006·Stem Cells·Catharina EllerströmHenrik Semb
Mar 5, 2008·Molecular Reproduction and Development·Shobhit SaxenaSatish Totey
Apr 17, 2008·The International Journal of Developmental Biology·Livia EiselleovaPetr Dvorak
Mar 11, 2011·Nature Reviews. Cancer·Uri Ben-David, Nissim Benvenisty
Mar 23, 2011·Journal of Cellular and Molecular Medicine·Hanae UeyamaKoji Kawakami
Nov 15, 2011·International Review of Cell and Molecular Biology·Elena S PhilonenkoSergey L Kiselev
Jan 28, 2012·Lancet·Steven D SchwartzRobert Lanza
Jun 15, 2013·Head & Neck·Nicole Y K LiSusan L Thibeault
Jan 1, 2013·Acta Medica (Hradec Králové)·Jakub SuchánekTomás Soukup
Dec 19, 2014·Thrombosis and Haemostasis·Praewphan IngrungruanglertVorasuk Shotelersuk

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Methods Mentioned

BETA
flow cytometry
SMA
enzymatic dissociation
fluorescence microscopy
PCR

Software Mentioned

SPSS

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