Human umbilical cord-derived mesenchymal stem cells suppress proliferation of PHA-activated lymphocytes in vitro by inducing CD4(+)CD25(high)CD45RA(+) regulatory T cell production and modulating cytokine secretion

Cellular Immunology
Hongna YangTingyu Qu

Abstract

Bone marrow-derived mesenchymal stem cells (MSCs) are promising candidate cells for therapeutic application in autoimmune diseases due to their immunomodulatory properties. Unused human umbilical cords (UC) offer an abundant and noninvasive source of MSCs without ethical issues and are emerging as a valuable alternative to bone marrow tissue for producing MSCs. We thus investigated the immunomodulation effect of umbilical cord-derived MSCs (UC-MSCs) on human peripheral blood mononuclear cells (PBMCs), T cells in particular, in a co-culture system. We found that UC-MSCs efficiently suppressed the proliferation of phytohaemagglutinin (PHA)-stimulated PBMCs (p<0.01). Kinetic analysis revealed that UC-MSCs primarily inhibited the division of generation 3 (G3) and 4 (G4) of PBMCs. In addition, UC-MSCs augmented the expression of CD127(+) and CD45RA(+) but reduced the expression of CD25(+) in PBMCs stimulated by PHA (p<0.05). Furthermore, UC-MSCs inhibited PHA-resulted increase in the frequency of CD4(+)CD25(+)CD127(low/-) Tregs significantly (p<0.01) but augmented PHA-resulted increase in the frequency of CD4(+)CD25(high)CD45RA(+) Tregs to about three times in PBMCs. The levels of anti-inflammatory cytokines, PEG2, TGF-β, and IL-10 ...Continue Reading

References

Aug 3, 2004·Nature Medicine·Anne O'Garra, Paulo Vieira
Jul 5, 2006·The Journal of Experimental Medicine·Weihong LiuJeffrey A Bluestone
Nov 1, 2007·European Journal of Immunology·Shimon SakaguchiMakoto Miyara
Dec 11, 2007·Stem Cells·Deryl L Troyer, Mark L Weiss
Mar 17, 2009·Clinical and Molecular Allergy : CMA·Subhadra NandakumarUday Kumaraguru
Sep 10, 2009·International Immunology·Shimon SakaguchiTomoyuki Yamaguchi
Mar 9, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Ke ChenZhong Chao Han
Apr 14, 2010·Immunology and Cell Biology·Shabari TipnisAnish S Majumdar
May 28, 2010·Stem Cell Research & Therapy·Soufiane GhannamDanièle Noël
Jun 19, 2010·Nature Reviews. Immunology·Shimon SakaguchiDavid A Hafler
Apr 12, 2011·Bone Marrow Transplantation·M E BernardoF Locatelli
Jul 8, 2011·Placenta·R R TaghizadehC L Cetrulo

❮ Previous
Next ❯

Citations

Aug 17, 2018·Current Stem Cell Research & Therapy·Mahboobeh RazmkhahAbbas Ghaderi
Jun 6, 2017·Bulletin of Experimental Biology and Medicine·A M PoltavtsevE V Svirshchevskaya
Jun 7, 2020·Stem Cell Research & Therapy·Ying LinXiaomin Chen
Dec 1, 2020·Frontiers in Cell and Developmental Biology·Annalisa AdamoMauro Krampera
Apr 24, 2021·Frontiers in Immunology·Qingyuan ZhengXiao-Kang Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.