Abstract
Bone marrow-derived mesenchymal stem cells (MSCs) are promising candidate cells for therapeutic application in autoimmune diseases due to their immunomodulatory properties. Unused human umbilical cords (UC) offer an abundant and noninvasive source of MSCs without ethical issues and are emerging as a valuable alternative to bone marrow tissue for producing MSCs. We thus investigated the immunomodulation effect of umbilical cord-derived MSCs (UC-MSCs) on human peripheral blood mononuclear cells (PBMCs), T cells in particular, in a co-culture system. We found that UC-MSCs efficiently suppressed the proliferation of phytohaemagglutinin (PHA)-stimulated PBMCs (p<0.01). Kinetic analysis revealed that UC-MSCs primarily inhibited the division of generation 3 (G3) and 4 (G4) of PBMCs. In addition, UC-MSCs augmented the expression of CD127(+) and CD45RA(+) but reduced the expression of CD25(+) in PBMCs stimulated by PHA (p<0.05). Furthermore, UC-MSCs inhibited PHA-resulted increase in the frequency of CD4(+)CD25(+)CD127(low/-) Tregs significantly (p<0.01) but augmented PHA-resulted increase in the frequency of CD4(+)CD25(high)CD45RA(+) Tregs to about three times in PBMCs. The levels of anti-inflammatory cytokines, PEG2, TGF-β, and IL-10 ...Continue Reading
References
Aug 3, 2004·Nature Medicine·Anne O'Garra, Paulo Vieira
Aug 24, 2004·Scandinavian Journal of Immunology·K Le BlancO Ringdén
Jul 5, 2006·The Journal of Experimental Medicine·Weihong LiuJeffrey A Bluestone
Aug 19, 2006·Blood·Petra HoffmannMatthias Edinger
Nov 1, 2007·European Journal of Immunology·Shimon SakaguchiMakoto Miyara
Dec 11, 2007·Stem Cells·Deryl L Troyer, Mark L Weiss
Apr 19, 2008·Stem Cells·Biju ParekkadanMartin L Yarmush
Dec 9, 2008·Journal of Autoimmunity·Imene BoumazaMaryam Feili-Hariri
Mar 17, 2009·Clinical and Molecular Allergy : CMA·Subhadra NandakumarUday Kumaraguru
Sep 10, 2009·International Immunology·Shimon SakaguchiTomoyuki Yamaguchi
Nov 27, 2009·The Journal of Investigative Dermatology·Sandra KleinAnnegret Kuhn
Mar 9, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Ke ChenZhong Chao Han
Apr 14, 2010·Immunology and Cell Biology·Shabari TipnisAnish S Majumdar
May 28, 2010·Stem Cell Research & Therapy·Soufiane GhannamDanièle Noël
May 28, 2010·Arthritis and Rheumatism·Lingyun SunSongtao Shi
Jun 19, 2010·Nature Reviews. Immunology·Shimon SakaguchiDavid A Hafler
Apr 12, 2011·Bone Marrow Transplantation·M E BernardoF Locatelli
Jul 8, 2011·Placenta·R R TaghizadehC L Cetrulo
Oct 19, 2011·Cellular Immunology·Changhui ZhouFangxia Guan
Jul 11, 2012·Cell Biochemistry and Function·Hao ZhangHu Chen
Jun 6, 2013·Stem Cell Research & Therapy·Patricia Luz-CrawfordFlavio Carrión
Aug 13, 2013·PloS One·Hongna YangJianzhong Bi
Citations
Apr 1, 2011·Journal of Translational Medicine·Wenxue MaBoris Minev
Nov 19, 2013·PloS One·Mark ReadVipin Kumar
Sep 16, 2014·Veterinary Immunology and Immunopathology·E L NaceD J Hurley
Sep 30, 2016·World Journal of Stem Cells·Oscar Ka-Fai Ma, Koon Ho Chan
Aug 17, 2018·Current Stem Cell Research & Therapy·Mahboobeh RazmkhahAbbas Ghaderi
Aug 6, 2019·Immunotherapy·Jose R LamasLuis Rodriguez-Rodriguez
Jun 6, 2017·Bulletin of Experimental Biology and Medicine·A M PoltavtsevE V Svirshchevskaya
Oct 23, 2019·Stem Cells Translational Medicine·Oula KhourySean V Murphy
Aug 31, 2017·Bulletin of Experimental Biology and Medicine·A M PoltavtsevE V Svirshchevskaya
Jun 27, 2020·Pharmaceutics·Marina O GomzikovaAlbert A Rizvanov
Jun 7, 2020·Stem Cell Research & Therapy·Ying LinXiaomin Chen
Dec 1, 2020·Frontiers in Cell and Developmental Biology·Annalisa AdamoMauro Krampera
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Stefania CrociCarlo Salvarani
Apr 24, 2021·Frontiers in Immunology·Qingyuan ZhengXiao-Kang Li
May 21, 2021·Frontiers in Cell and Developmental Biology·Yufeng ShangFuling Zhou
Sep 17, 2021·Journal of Ovarian Research·Xiaodan LvXu Ma