Human umbilical cord mesenchymal stem cells-derived exosomes deliver microRNA-375 to downregulate ENAH and thus retard esophageal squamous cell carcinoma progression.

Journal of Experimental & Clinical Cancer Research : CR
Zhanfeng HeSong Zhao

Abstract

Exosomal microRNAs (miRNAs or miRs) from bone marrow-derived mesenchymal stem cells (UCMSCs) have emerged as promising therapeutic strategies for cancer treatment. The current study aimed to elucidate the underlying mechanism of human umbilical cord mesenchymal stem cells (hUCMSCs)-derived exosomal miR-375 in esophageal squamous cell carcinoma (ESCC). After determining the expression of miR-375 and its putative target enabled homolog (ENAH) in ESCC tissues and cells, we tested effects of their altered expression on ESCC proliferation, invasion, migration, and tumorsphere formation was subsequently measured. Transfected hUCMSCs-derived exosomes (hUCMSCs-exo) were isolated and co-cultured with ESCC cells to measure the effects of miR-375 delivered by hUCMSCs-exo on ESCC development. Finally, we investigated the effect of miR-375 on tumor growth in vivo. The expression of miR-375 was reduced, while the expression of ENAH was elevated in ESCC. ENAH was identified as a target gene of miR-375. Elevated miR-375 or depleted ENAH expression inhibited ESCC cell proliferation, invasion, migration, tumorsphere formation, and promoted apoptosis. Moreover, miR-375 delivered by hUCMSCs-exo could suppress ESCC cell proliferation, invasion, mig...Continue Reading

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Citations

Oct 8, 2020·International Journal of Molecular Sciences·Catharina MelzerRalf Hass
Oct 20, 2020·Journal of Dental Research·H Z ShiA D Le
Dec 29, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Yu-Shui MaDa Fu
Mar 9, 2021·Frontiers in Oncology·Xueyang CuiYuan Yuan
Apr 19, 2021·Seminars in Cell & Developmental Biology·Priya MondalSyed Musthapa Meeran
Aug 12, 2021·Biotechnology Reports·Makalakshmi MuralikumarAntara Banerjee

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Datasets Mentioned

BETA
GSE55856
GSE20347
GSE29001
GSE6188
GPL4508
GPL14613
GSE97320
GPL571
DIANA

Methods Mentioned

BETA
surgical resection
transfection
Assay
flow cytometry
Cesarean section
PCR
xenograft

Software Mentioned

TargetScan
Affymetrix R
ImageJ
limma

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