Human Urine-Derived Stem Cell Differentiation to Endothelial Cells with Barrier Function and Nitric Oxide Production

Stem Cells Translational Medicine
Guihua LiuYuanyuan Zhang

Abstract

Endothelial cells (ECs) play a key role in revascularization within regenerating tissue. Stem cells are often used as an alternative cell source when ECs are not available. Several cell types have been used to give rise to ECs, such as umbilical cord vessels, or differentiated from somatic stem cells, embryonic, or induced pluripotent stem cells. However, the latter carry the potential risk of chronic immune rejection and oncogenesis. Autologous endothelial precursors are an ideal resource, but currently require an invasive procedure to obtain them from the patient's own blood vessels or bone marrow. Thus, the goal of this study was to determine whether urine-derived stem cells (USCs) could differentiate into functional ECs in vitro. Urine-derived cells were then differentiated into cells of the endothelial lineage using endothelial differentiation medium for 14 days. Changes in morphology and ultrastructure, and functional endothelial marker expression were assessed in the induced USCs in vitro. Grafts of the differentiated USCs were then subcutaneously injected into nude mice. Induced USCs expressed significantly higher levels of specific markers of ECs (CD31, vWF, eNOS) in vitro and in vivo, compared to nondifferentiated USC...Continue Reading

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Citations

Aug 17, 2018·Stem Cells Translational Medicine·Stuart P Atkinson
Nov 28, 2018·Journal of Cellular Physiology·Chunyu HeHuirong Liu
Jun 4, 2019·Journal of Cardiovascular Translational Research·Ampadu O JacksonKai Yin
Jan 11, 2020·Frontiers in Molecular Neuroscience·Mitsuto SatoYoshitsugu Aoki
Aug 28, 2020·Frontiers in Cell and Developmental Biology·Min LiuRong Zhou
Nov 13, 2020·World Journal of Stem Cells·Perrine BurdeyronClara Steichen
Jun 6, 2020·Biomedical Materials·Igor VasyutinYuanyuan Zhang
Sep 7, 2021·Frontiers in Bioengineering and Biotechnology·Ifeolu AkinnolaAngela Panoskaltsis-Mortari
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Jan 21, 2022·Tissue Engineering. Part B, Reviews·Yi-Zhou HuangHui-Qi Xie

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Methods Mentioned

BETA
coronary
peripheral artery bypass
flow cytometry
Polymerase Chain Reaction
PCR
Assay
phase contrast microscopy
ELISA
urine collection
acetylation

Software Mentioned

ImagePro
Quantity One
Image J

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