Human uterine leiomyoma contains low levels of 1, 25 dihdroxyvitamin D3, and shows dysregulated expression of vitamin D metabolizing enzymes

European Journal of Obstetrics, Gynecology, and Reproductive Biology
Essam R OthmanAlaa-Eldin A Yousef

Abstract

To evaluate tissue concentration of 1, 25 dihydroxyvitamin D3, and gene expression level of CYP27B1 that codes for 1-α hydroxylase (vitamin D activating enzyme), and CYP24A1 that codes for 24-hydroxylase (vitamin D catabolizing enzyme) in human uterine leiomyoma (ULM), its adjacent myometrium (Myo-F), and normal myometrium (Myo-N). Levels of 1, 25 dihydroxyvitamin D3 were measured using HPLC and Diode detectors whereas CYP27B1, and CYP24A1 expressions were assessed using Real-Time PCR in ULM, Myo-F, and Myo-N. Non-parametric statistics were used. ULMs contained significantly less 1, 25 dihydroxy vitamin D3 compared to Myo-F (3.0, IQR: 1.0-9.0 versus 6.0, IQR: 3.0-13.0 μg/ kg, P value is 0.03). No significant difference was detected between ULM and Myo-N, or Myo-F and Myo-N. Intratumoral level of the active form of vitamin D did not differ according to the type of ULM (submucous or interstitial/subserous), or to the ULM volume. CYP27B1 was expressed in ULM (2.17, IQR: 0.65-4.9), Myo-F (4.94, IQR: 1.04-22.59), and Myo-N (0.99, IQR: 0.49-1.71) to a comparable level. CYP24A1 expression was significantly higher in ULM compared to Myo-N (2.00, IQR: 0.69-10.77 versus 0.22, IQR: 00- 0.96, respectively, P value is 0.04). Human ULMs cont...Continue Reading

Citations

Jan 12, 2019·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Michał CiebieraGrzegorz Jakiel
Nov 21, 2019·Journal of Clinical Laboratory Analysis·Elnaz FazeliReza Mirfakhraie
Oct 8, 2020·Archives of Gynecology and Obstetrics·Elnaz FazeliReza Mirfakhraie
Oct 28, 2020·Reproductive Sciences·Michał CiebieraAyman Al-Hendy
Mar 7, 2021·Nutrients·Daniele VergaraAndrea Tinelli

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