Human vascular smooth muscle cells from diabetic patients are resistant to induced apoptosis due to high Bcl-2 expression

Diabetes
Emilio RuizTeresa Tejerina

Abstract

An emerging body of evidence suggests that vascular remodeling in diabetic patients involves a perturbation of the balance between cell proliferation and cell death. Our aim was to study whether arteries and vascular smooth muscle cells (VSMCs) isolated from diabetic patients exhibit resistance to apoptosis induced by several stimuli. Internal mammary arteries (IMAs) were obtained from patients who had undergone coronary artery bypass graft surgery. Arteries from diabetic patients showed increasing levels of Bcl-2 expression in the media layer, measured by immunofluorescence and by Western blotting. Human IMA VSMCs from diabetic patients showed resistance to apoptosis, measured as DNA fragmentation and caspase-3 activation, induced by C-reactive protein (CRP) and other stimuli, such as hydrogen peroxide and 7beta-hydroxycholesterol. The diabetic cells also exhibited overexpression of Bcl-2. Knockdown of Bcl-2 expression with Bcl-2 siRNA in cells from diabetic patients reversed the resistance to induced apoptosis. Consistent with the above, we found that pretreatment of nondiabetic VSMCs with high glucose abolished the degradation of Bcl-2 induced by CRP. Moreover, cell proliferation was increased in diabetic compared with nondi...Continue Reading

References

Jul 1, 1988·Journal of the American College of Cardiology·J A AmbroseV Fuster
Jan 1, 1981·Clinical Pharmacology and Therapeutics·M S Kramer, A R Feinstein
May 1, 1995·The Journal of Clinical Investigation·M R BennettS M Schwartz
May 19, 1994·The New England Journal of Medicine·G H Gibbons, V J Dzau
Apr 1, 1996·The Journal of Clinical Investigation·V V KunjathoorR C LeBoeuf
Jul 11, 2002·American Journal of Physiology. Cell Physiology·Hiroya SakumaKeigo Yasuda
Nov 16, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Chhinder P SodhiAtul Sahai
Apr 16, 2003·Circulation·Ronald M A HenryUNKNOWN Hoorn Study
May 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yili Yang, Xiaodan Yu
Jun 21, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Ashley S IzzardAnthony M Heagerty
Jul 8, 2003·The Journal of Physiology·Ana M BrionesSilvia M Arribas
Feb 14, 2004·Stroke; a Journal of Cerebral Circulation·Ronald M A HenryCoen D A Stehouwer
Dec 25, 2004·Diabetes Care·UNKNOWN American Diabetes Association
Apr 2, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Karolina E TaylorCarmen W van den Berg
Apr 26, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Cauveh EramiJames E Faber
Jul 28, 2005·Diabetes·Elena B OkonCornelis van Breemen

❮ Previous
Next ❯

Citations

Apr 9, 2008·Current Opinion in Hematology·Vyacheslav A Korshunov, Bradford C Berk
Sep 4, 2013·International Journal of Obesity : Journal of the International Association for the Study of Obesity·F J OrtegaJ M Fernández-Real
Mar 18, 2011·Journal of Cellular Physiology·Hui ChangMan-Jiang Xie
May 26, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kok Meng ChanSalmaan Hussain Inayat-Hussain
Nov 1, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Gopal V Velmurugan, Carl White
Mar 27, 2018·Journal of Cellular Physiology·Tran T HienSebastian Albinsson

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.