Human yolk-sac tumor antigen 2G10: biochemical characterization and significance as a serum antigen.

International Journal of Cancer. Journal International Du Cancer
S TanakaJ Hata

Abstract

The biochemical characterization of human yolk-sac tumor (YST) antigen 2G10, detected by monoclonal antibody (MAb) M912-2G10, was studied. Previous results indicated that glycolipids having a non-reducing terminal N-acetyllactosamine structure were the epitope of 2G10 on human erythrocytes. In this study, the glycoprotein nature of 2G10 on the infantile embryonal carcinoma line, MTE, was investigated. 2G10 activity, measured by a new enzyme-linked immunosorbent assay (2G10-ELISA), was recovered in residual fractions of MTE from which glycolipids were removed. Chromatographically, 3H-galactose-labelled 2G10 on MTE had a molecular weight (mw) of about 580 kDa, which decreased after pronase or alkaline-borohydride treatment. Our results indicate the glycoprotein nature of 2G10 on MTE. Furthermore, 2G10, both on erythrocytes and on MTE, was sensitive to galactosidase but not to neuraminidase and fucosidase, suggesting that terminal galactose is involved in the antigenic structure. It was also found by 2G10-ELISA that 2G10 sheds from tumor cells. Shedding occurs in nude mice transplanted with MTE as well as in patients with germ-cell tumors (GCTs). The serum level of 2G10 in non-tumor patients was low, but high levels were detected ...Continue Reading

References

Oct 18, 1979·Biochimica Et Biophysica Acta·T MuramatsuF Jacob
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B Mintz, K Illmensee
Jun 1, 1985·Cancer·N J VogelzangE F Fraley
Mar 2, 1982·Biochemistry·K HofmannF M Finn

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